Plant research covers a variety of practical applications, such as plant identification, diagnosis of plant pathogens, genetic variation measurement, plant improvement, plant transformation, etc. The process of plant research requires the choice of proper protocols, so do the corresponding reagents, such as plant nucleic acid isolation, PCR/ qRT-PCR, gel electrophoresis, cloning, sequencing, western blot, etc. TransGen provides integrated plant research related reagents to push your science forward.
DNA/RNA Extraction Reagents in Plant Research
DNA/RNA extraction is usually the first step required for most molecular biology investigations in plant research. Plants present some unique challenges compared to animal tissues in DNA/RNA extraction. For example, the tough cell wall of many plant tissues often requires time-consuming enzymatic or mechanical techniques to break apart. In addition, other problems may be encountered including the presence of large quantities of polysaccharides; high levels of RNases; various different kinds of phenolics, including tannins; low concentrations of nucleic acids (high water content); tissue, such as lignin (wood), that is difficult to break up; and so on. To overcome these problems and difficulties, TransGen provides both silica-based spin column method and modified CTAB method tailored for plant DNA/RNA extraction. Furthermore, soil DNA extraction reagent based on magnetic bead method is also available.
★ Plant DNA extraction
• Silica based spin column method: EasyPure® Plant Genomic DNA Kit (Cat. No. EE111)
• Modified CTAB method: PlantZol (Cat. No. EE141)
★ Plant RNA extraction
• Silica based spin column method: EasyPure®Plant RNA Kit (Cat. No. ER301)
• Modified CTAB method: TransZol Plant (Cat. No. ET121)
★ DNA extraction from soil samples
• Magnetic bead based method: MagicPure® Stool and Soil Genomic DNA Kit (Cat. No. EC801)
Learn more about DNA/RNA Extraction Reagents in Plant Research〉
PCR Reagents in Plant Research
The polymerase chain reaction (PCR) is one of the most useful and widely used protocols in applied plant research . TransGen, based on abundant technical experience, can provide high-quality PCR reagents with high fidelity and high specificity for DNA amplification in plant research.
• PerfectStart™ Taq DNA Polymerase (Cat. No. AP401)
• TransStart® FastPfu DNA Polymerase(Cat. No. AP221)
• TransStart® FastPfu Fly DNA Polymerase(Cat. No. AP231)
Scan the full list of PCR reagents for plant research 〉
Direct PCR Reagents in Plant Research
Conventional PCR requires purified DNA molecules as templates. Purification of DNA molecules from a large number of samples is laborious, costly and time-consuming. Therefore, various direct-PCR methods using tissues directly employed as templates have been developed. TransGen provides a direct PCR reagent tailored for plant samples to boost your research.
• TransDirect® Plant Tissue PCR Kit (Cat. No.AD301)
√ Direct PCR amplification from lysate prepared using proprietary formulated lysis reagent.
√ Suitable for high throughput applications.
√ Not suitable for plant tissues rich in polysaccharide and polyphenol.
Real time PCR/RT-PCR Reagents in Plant Research
Quantitative real-time polymerase chain reaction (qRT-PCR) is a commonly used and powerful tool for accurately analysing gene expression levels in different samples or under different experimental conditions in a wide range of biological experiments. TransGen supplies excellent reverse transcription reagents and real time PCR reagents to cultivate your plant research.
• PerfectStart™ Green qPCR SuperMix (Cat. No. AQ601)
• TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR(One-Step gDNA Removal) (Cat. No. AT341)
• TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal) (Cat. No. AH341)
Learn more about real time PCR/ RT-PCR reagents for plant research 〉
DNA Gel Electrophoresis Reagents in Plant Research
Agarose gel electrophoresis is the simplest method for the general analysis of plant DNA. This technique reveals the size distribution of DNA in any sample together with the approximate concentrations of the DNA components in the sample. The concentration of agarose in the gel can be varied to separate DNA molecules of different sizes. Polyacrylamide gel electrophoresis provides greater resolution than agarose gel electrophoresis in the analysis of small DNA fragments but is generally more difficult to perform. TransGen supplies DNA Markers, Gelstain and Agarose to support your gel electrophoresis in plant research.
• DNA Markers
• GelStain
• Agarose
Gene Cloning Reagents in Plant Research
Cloning of PCR products is a common requirement in the production of gene constructs, useful probes and the sequencing of some PCR products. Blunt cloning has been widely used to clone PCR products, which is more suitable for blunt ended products. For products with a 3'-A overhang, vectors with a complementary T can be used to clone these products with high efficiency, which is known as TA cloning. TransGen not only provides cloning vectors for blunt cloning and TA cloning, but also seamless cloning vectors. Furthermore, Cloning competent cells with high transformation efficiency are also available.
• pEASY Cloning Vectors
√ Fast: 5 minutes at room temperature.
√ Simple: only add PCR products.
√ High efficiency: up to 90% clones with correct insert.
• Cloning Competent Cells
Gene Expression Reagents in Plant Research
Cloning in expression vectors may be used to generate large amounts of plant proteins in bacteria. These proteins may be useful in food or industrial processes or may be used in characterizing the protein. This approach may be of special value when the protein is not abundant in the plant or is difficult to purify. TransGen supplies prokaryotic expression vectors and expression competent cells to push forward your plant research.
• pEASY Prokaryotic Expression Vectors
• Expression Competent Cells
NGS Library Construction Reagents in Plant Research
Next generation sequencing (NGS) technologies have opened fascinating opportunities for the analysis of plants. During the last few years, NGS methods have become widely available and cost effective. They can be applied to a wide variety of biological questions, from the sequencing of complete eukaryotic genomes and transcriptomes, to the genome-scale analysis of DNA-protein interactions. High-efficiency library preparation methods seems to be necessary and important for the process. TransGen provides a series of products for NGS library construction, including library preparation kit, rRNA depletion, DNA size selection……
• TransNGS® Tn5 DNA Library Prep Kit for Illumina® (for 50 ng DNA) (Cat. No. KP101)
•TransNGS® DNA Library Prep Kit for Illumina®(Cat. No. KP201)
Scan the full list of NGS Library Construction Products in Plant Research〉
Western Blot & Immunofluorescence (IF) Reagents in Plant Research
Western blot (WB) is a widely used immunological technique in plant research to detect specific proteins in tissue homogenate or extract. Native proteins are separated through gel electrophoresis and then transferred to a membrane, where target proteins are detected by specific antibodies. WB can detect nano-gram amount of target proteins using high quality antibodies.
Immunofluorescence light microscopy has made a significant impact on plant research over the past decade. It is a powerful yet convenient tool, as it offers all the advantages of the conventional light microscope but with increased specificity and sensitivity. It has found application in numerous investigations of plant cells, particularly those related to the plant cytoskeleton. Immunofluorescence light microscopy permits the rapid examination of the three-dimensional organization of microtubules (MTs) in fixed whole cells of plants.
TransGen supplies validated tag antibodies and secondary antibodies for western blot and Immunofluorescence (IF) assays in plant research. Protein markers, chemiluminescent substrate, and precast gel are also provided to support your western blot experiments.
• Tag Antibodies
• Secondary Antibodies
• Western Protein Markers
• Chemiluminescent Substrate for Western Blot
• Precast Tris-Glycine Gel
References
Henry R J. Practical applications of plant molecular biology[M]. Garland Science, 1997.
Tamari F, Hinkley C S. Extraction of DNA from plant tissue: review and protocols[M]//Sample preparation techniques for soil, plant, and animal samples. Humana Press, New York, NY, 2016: 245-263.
Yockteng R, Almeida A M R, Yee S, et al. A method for extracting high‐quality RNA from diverse plants for next‐generation sequencing and gene expression analyses[J]. Applications in plant sciences, 2013, 1(12): 1300070.
Bräutigam A, Gowik U. What can next generation sequencing do for you? Next generation sequencing as a valuable tool in plant research. Plant Biol (Stuttg). 2010 Nov;12(6):831-41.
RONG R, WU P, LAN J, et al. Western blot detection of PMI protein in transgenic rice[J]. Journal of integrative agriculture, 2016, 15(4): 726-734.
Wang H, Fowke L C. Indirect Immunofluorescence Techniques for Cultured Plant Cells and Protoplasts[M]//Plant Cell, Tissue and Organ Culture. Springer, Berlin, Heidelberg, 1995: 239-250.
