PCR Enzymes
  • AP231-01.JPG
AP231-01.JPG

TransStart® FastPfu Fly DNA Polymerase


Product Details

TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. TransStart® FastPfuFly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.

TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 2-6 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

Applications
• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAP231-01/11AP231-02/12AP231-03/13
TransStart® FastPfu Fly DNA Polymerase250 U×1500 U×1500 U×6
TransStart® FastPfu Fly Buffer1.2 ml×11.2 ml ×21.2 ml ×12
2.5 mM dNTPs- / 500 μl×1- / 1 ml ×1- / 1 ml ×6
50 mM MgSO4200 μl×1400 μl ×11 ml ×1
PCR Stimulant200 μl×1400 μl×11 ml ×1
6×DNA Loading Buffer500 μl×11 ml ×11 ml ×2


Citations

Zhu X J , Sun S , Xie B , et al. Guanine-rich sequences inhibit proofreading DNA polymerases[J]. Scientific Reports, 2016, 6:28769.

Ye F , Lan X E , Zhu W B , et al. Mitochondrial genomes of praying mantises (Dictyoptera, Mantodea): rearrangement, duplication, and reassignment of tRNA genes[J]. Scientific Reports, 2016, 6:25634.

Hu L , Li H , Qin R , et al. Plant phosphomannose isomerase as a selectable marker for rice transformation[J]. Scientific Reports, 2016, 6(1):25921.

Chao H , Zhou N Y . GenR, an IclR-Type Regulator, Activates and Represses the Transcription of gen Genes Involved in 3-Hydroxybenzoate and Gentisate Catabolism in Corynebacterium glutamicum[J]. Journal of Bacteriology, 2013, 195(7):1598-1609.

Liu X, Pu J, Zeng S, et al. Hyriopsis cumingii Hic52-A novel nacreous layer matrix protein with a collagen-like structure[J].Int J Biol Macromol. 2017 Sep;102:667-673.

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