RNA Extraction for SARS-CoV-2 Detection

Manual RNA extraction

·· Spin column-based: EasyPure^® Viral DNA/RNA Kit (Cat. No. ER201)

·· Magnetic bead-based: MagicPure® Viral DNA/RNA Kit (Cat. No. EC301)

Automated RNA extraction 

·· Automated nucleic acid extractor:  TS-32 Automated Nucleic Acid Extractor 

                                                                                   TS-96 Automated Nucleic Acid Extractor   

·· Pre-filled nucleic acid extraction kit:  MagicPure^® 32 Viral DNA/RNA Kit (Cat. No. EC301-32) 

                                                                                          MagicPure^® 96 Viral DNA/RNA Kit (Cat. No. EC301-96)                                         

SARS-CoV-2 Real Time PCR Detection Kit

··  SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex Real Time RT-PCR)

• High sensitivity: Limit of detection (LoD) of 0.5 copies/μl.

• High specificity: Proprietary Triple Antibody Blocking-based Hot Start Technology. No cross reactivity with HCoV-HKU1, HCoV-229E, HCoV-OC43 and HCoV-NL63.

• Fast workflow: Less than 60 min.

• Multiple validated instruments: ABI 7500, ABI QuantStudio 3, and Bio-Rad CFX96.

··  Trans SARS-CoV-2 & Influenza A/B Assay

• 3 tests in 1 setup: SARS-CoV-2, Influenza A virus and Influenza B virus

• High sensitivity: Limit of detection (LoD) of 0.5 copies/μl

• High specificity: Evaluation for cross-reactivity with multiple species

• Fast workflow: Completed in 65 min (reaction setup & RT-PCR run)

• Minimized contamination : dUTP/ UNG used

• CE marked

SARS-CoV-2 Real Time PCR Detection Instruments

·· AZURE CIELO™ Real-Time PCR System

Background Information for SARS-CoV-2 Detection

Coronavirus diseases 2019 (COVID-19) is a highly infectious and an emerging respiratory disease caused by a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 has a nonsegmented, linear, positive-sense, multicistronic genome and produces enveloped virions. The virus is classified as a betacoronavirus (Nidovirales: Coronaviridae) together with the other 2 highly virulent human pathogens severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV). The SARS-CoV-2 genome shares 79.6% and 50.0% nucleotide sequence identity with the genomes of SARS-CoV and MERS-CoV, respectively. Similar to SARS-CoV, SARS-CoV-2 virions use their spike (S) glycoproteins to engage host-cell angiotensin I–converting enzyme 2 (ACE2) to gain entry into host cells and host-cell transmembrane serine protease 2 (TMPRSS2) for S priming.

Diagnostics can play an important role in the containment of COVID-19, enabling rapid implementation of control measures that limit the spread through case identification, isolation, and contact tracing (i.e., identifying people that may have come in contact with an infected patient). Molecular techniques are more suitable for accurate diagnoses because they can target and identify specific pathogens. The most commonly used targets for primer/probe development in case of SARS-CoV-2 are from conserved viral genome including ORF1ab gene, RdRP gene (RNA dependent RNA polymerase), N gene (Nucleopcapsid) and E gene (Envelope). Real-time reverse transcription-polymerase chain reaction (qRT-PCR) of upper respiratory specimens, such as nasopharyngeal swabs and pharyngeal swabs, has been widely used to confirm the clinical diagnosis of COVID-19.

References

Liu J, Babka AM, Kearney BJ, Radoshitzky SR, Kuhn JH, Zeng X. Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens. JCI Insight. 2020;5(12):e139042.

Bwire GM, Majigo MV, Njiro BJ, Mawazo A. Detection profile of SARS-CoV-2 using RT-PCR in different types of clinical specimens: A systematic review and meta-analysis [published online ahead of print, 2020 Jul 24]. J Med Virol.

SARS-CoV-2 Detection Related Pages

Selected Real Time PCR/ RT-PCR Reagents Solutions      One Stop from Viral Nucleic Acid Extraction to Detection