Viruses are very tiny organisms, but sometimes they can cause big troubles. One of the most famous ones is SARS-CoV-2, which has led to tremendous loss to the whole world. Actually, there are numerous diseases due to virus such as the common cold and flu, and severe illnesses such as HIV/AIDS, smallpox, and Ebola.
To handle virus infection diseases and save people’s life timely, efficient diagnosis is necessary. For laboratory-based diagnosis, real-time (quantitative) PCR assays, which is now widely used to detect viral nucleic acids in clinical specimens, facilitate the very rapid, sensitive, and specific identification of many known pathogenic viruses, and automation of these assays allows the processing of large numbers of samples in short periods of time (high sample-throughput).
Based on years of experience on nucleic acid extraction and PCR technology, TransGen Biotech provides one-stop solutions for real time PCR virus detection. Get started to scan multiple viral nucleic acid extraction and real time PCR detection products tailored for virus detection research applications
Viral nucleic acid extraction
After a viral sample is collected, it will be treated by nucleic acid extraction which is imperative in delivering intact, un-damaged and contamination-free starting materials for the very sensitive method of polymerase chain reaction (PCR). 3 effective methods are recommended here for viral nucleic acid extraction:
1, Manual spin column-based nucleic acid extraction:EasyPure® Viral DNA/RNA Kit (Cat. No. ER201)
2, Manual magnetic beads nucleic acid extraction:MagicPure® Viral DNA/RNA Kit (Cat. No. EC301)
3, Automated nucleic acid extraction
(1) Pre-filled 32-well nucleic acid extraction kit:MagicPure® 32 Viral DNA/RNA Kit (Cat. No. EC301-32)
(2) Pre-filled 96-well nucleic acid extraction kit:MagicPure® 96 Viral DNA/RNA Kit (Cat. No. EC301-96)
To learn more about Viral nucleic acid extraction
Viral nucleic acid detection using real time PCR
The genetic material of the virus can be either DNA or RNA. For DNA viruses including the adenoviruses, herpesviruses, polyomaviruses and bocaviruses, the extracted DNA can be directly detected by real time PCR. On the other hand, for RNA viruses including coronaviruses, Zika virus, dengue viruses and Ebola virus, the extracted RNA needs to be converted to DNA before detected by real time PCR or directly detected by real time RT-PCR kits.
1.Real time PCR kit used for virus DNA detection or virus RNA detection after reverse transcription
PerfectStart™ II Probe qPCR SuperMix (Cat. No. AQ711)
2.Real time RT-PCR kits for RNA virus detection
TransScript® II Probe One-Step qRT-PCR SuperMix (Cat. No. AQ321)
TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG (Cat. No. AQ322)
Product summary for viral nucleic acid extraction and detection
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