Real-time qPCR is a PCR method used to amplify and simultaneously quantify target DNA molecules. Two methods are
frequently used for qPCR: double-strand DNA-binding dyes (e.g., SYBR® Green I) or fluorescent reporter probes (e.g., TaqMan®).
In both cases, fluorescence signals are detected during the exponential phase.
Real-time qPCR is a PCR method used to amplify and simultaneously quantify target DNA molecules. Two methods are
frequently used for qPCR: double-strand DNA-binding dyes (e.g., SYBR® Green I) or fluorescent reporter probes (e.g., TaqMan®).
In both cases, fluorescence signals are detected during the exponential phase.