qPCR and qRT-PCR SuperMixes
  • AQ141-01.JPG
AQ141-01.JPG

TransStart® Tip Green qPCR SuperMix


Product Details

TransStart® Tip Green qPCR SuperMix is a ready-to-use qPCR cocktail. It contains a novel TransStart® TipTaq DNA Polymerase,unique hot start reagents (DNA binding proteins combined with unique chemical), optimized double cation buffer, EvaGreen I, dNTPs, PCR Enhancerm and PCR stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and Nuclease-free Water.


Highlights

• A combination of chemical blocking technique with TransStart® hot start technique to achieve complete blocking. Compared with double blocking TransStart®TopTaq, this method provides higher sensitivity, higher specificity, better amplification.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.

Storage
at -20°C  in dark for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAQ141-01AQ141-02AQ141-03AQ141-04
TransStart® Tip Green qPCR SuperMix1 ml5×1 ml15×1 ml25×1 ml
Passive Reference Dye (50×)40 μl200 μl600 μl1 ml
Nuclease-free Water1 ml5 ml3×5 ml5×5 ml


Citations

Wei Y , Hu W , Wang Q , et al. Identification, transcriptional and functional analysis of heat-shock protein 90s in banana (Musa acuminata L.) highlight their novel role in melatonin-mediated plant response to Fusarium wilt[J]. Journal of Pineal Research, 2017, 62(1):e12367.

Wei Q , Jiao C , Guo L , et al. Exploring key cellular processes and candidate genes regulating the primary thickening growth of Moso underground shoots[J]. New Phytologist, 2017, 214(1):81-96.

Qi F , Yang J , Xia Y , et al. A(H1N1) vaccination recruits T lymphocytes to the choroid plexus for the promotion of hippocampal neurogenesis and working memory in pregnant mice[J]. Brain Behavior & Immunity, 2016, 53:72-83.

Liu G , Zhang K , Ai J , et al. Patatin-related phospholipase A, pPLAIIIα, modulates the longitudinal growth of vegetative tissues and seeds in rice[J]. Journal of Experimental Botany, 2015, 66(21):6945-6955.

Li P , Shi M L , Shen W L , et al. Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions[J]. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2017, 1860(8).

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