qPCR and qRT-PCR SuperMixes
  • AQ311-01.JPG
AQ311-01.JPG

TransScript®  II Green One-Step qRT-PCR SuperMix


Product Details

TransScript® II Green One-Step qRT-PCR SuperMix is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers to achieve one step from reverse transcription to qPCR in a single tube.


Highlights

TransScript® II Green One-Step RT/RI Enzyme Mix is used to efficiently synthesize first-strand cDNA from RNA and 2×PerfectStartTM Green One-Step qPCR SuperMix is used for qPCR, enabling simple procedures to minimize the chance of contamination.
High sensitivity and high specificity to ensure accurate results.


Applications
High-copy and low-copy gene detection
• Detection of RNA virus or trace amounts of RNA


Storage
at -20°C away from light for two years


Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAQ311-01AQ311-02
TransScript® II Green One-Step RT/ RI Enzyme Mix40 μl160 μl
PerfectStart® Green One-Step qPCR SuperMix (2×)1 ml4×1 ml
Passive Reference Dye (50×)40 μl160 μl
RNase-free Water1 ml4×1 ml


Citations

Du C , Wang Y , Zhang F , et al. Synaptotagmin-11 inhibits cytokine secretion and phagocytosis in microglia[J]. Glia, 2017, 65(10):1656-1667. 

Xiao T , Wang Y , Luo H , et al. A differential sequencing-based analysis of the C. elegans noncoding transcriptome[J]. RNA, 2012, 18(4):626-639.

Sleumer M C , Wei G , Wang Y , et al. Regulatory elements of Caenorhabditis elegans ribosomal protein genes[J]. BMC Genomics, 2012, 28(13):433.4.The 

loss of MiR-139-5p promotes colitis-associated tumorigenesis by mediating PI3K/AKT/Wnt signaling[J]. The International Journal of Biochemistry & Cell Biology, 2015, 69:153-161.

Aqian L , Guifeng W , Yunfei W , et al. Identification of Intermediate-Size Non-Coding RNAs Involved in the UV-Induced DNA Damage Response in C. elegans[J]. PLoS ONE, 2012, 7(11):e48066.

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