Product Details
TransScript® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for the detection and quantification of miRNA from total RNA, small RNA or other miRNA containing samples. TransScript® miRNA RT Enzyme Mix (Poly(A) polymerase and reverse transcriptase are included) and 2×TS miRNA Reaction Mix are supplied to efficiently add Poly(A) tails and synthesize first-strand cDNA. PerfectStart® Green qPCR SuperMix is used for miRNA quantification.
| Component | AQ202-01 |
| TransScript® miRNA RT Enzyme Mix | 20 μl |
| 2×TS miRNA Reaction Mix | 200 μl |
| Universal miRNA qPCR Primer (10 μM) | 200 μl |
| 2×PerfectStartTM Green qPCR SuperMix | 5×1 ml |
| Passive Reference Dye (50×) | 200 μl |
| RNase-free Water | 1 ml |
1 Yang Y, Lin Z, Han Z, et al. miR-539 activates the SAPK/JNK signaling pathway to promote ferropotosis in colorectal cancer by directly targeting TIPE[J]. Cell Death Discovery, 2021.(IF 5.24)
2 Hong Y H, Meng J, He X L, et al. Overexpression of MiR482c in tomato induces enhanced susceptibility to late blight[J]. Cells, 2019.(IF 5.65)
