qPCR and qRT-PCR SuperMixes
  • AQ202-01.JPG
AQ202-01.JPG

TransScript® Green miRNA Two-Step qRT-PCR SuperMix


Product Details

TransScript® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for the detection and quantification of miRNA from total RNA, small RNA or other miRNA containing samples. TransScript® miRNA RT Enzyme Mix (Poly(A) polymerase and reverse transcriptase are included) and 2×TS miRNA Reaction Mix are supplied to efficiently add Poly(A) tails and synthesize first-strand cDNA. PerfectStartTM Green qPCR SuperMix is used for miRNA quantification.


Highlights

• Optimal ratio of Poly(A) polymerase to reverse transcriptase and optimized reaction buffer, so as to ensure high transcription efficiency of miRNA.

• One-step completion of Poly(A) tail addition and first-strand cDNA synthesis in a single tube.

• High amplification efficiency, specificity and sensitivity ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.

• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).


Applications
Multiple copy and low copy gene detection.

Storage
at -20°C  in dark for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAQ202-01
TransScript® miRNA RT Enzyme Mix20 μl
2×TS miRNA Reaction Mix200 μl
Universal miRNA qPCR Primer (10 μM)200 μl
PerfectStartTM Green qPCR SuperMix5×1 ml
Passive Reference Dye (50×)200 μl
RNase-free Water1 ml


Citations

Zhang S , Zeng Y , Yi X , et al. Selection of suitable reference genes for quantitative RT-PCR normalization in the halophyte Halostachys caspica under salt and drought stress[J]. Scientific Reports, 2016, 6:30363.

Wu H , Li Z , Yang P , et al. PKM2 depletion induces the compensation of glutaminolysis through β-catenin/c-Myc pathway in tumor cells[J]. Cellular Signalling, 2014, 26(11):2397-2405.

The loss of MiR-139-5p promotes colitis-associated tumorigenesis by mediating PI3K/AKT/Wnt signaling[J]. The International Journal of Biochemistry & Cell Biology, 2015, 69:153-161.

Qiao Y , Mao Y , Wang J , et al. Analysis of liver and gill miRNAs of Larimichthys crocea against Cryptocryon irritans challenge[J]. Fish & Shellfish Immunology, 2016, 59:484-491.

Sun Y , Qiu Y , Zhang X , et al. Genome-wide identification of microRNAs associated with taproot development in radish (Raphanus sativus L.)[J]. Gene, 2015, 569(1):118-26.

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