qPCR and qRT-PCR SuperMixes
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TransScript® Green miRNA Two-Step qRT-PCR SuperMix

Product Details

TransScript® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for the detection and quantification of miRNA from total RNA, small RNA or other miRNA containing samples. TransScript® miRNA RT Enzyme Mix (Poly(A) polymerase and reverse transcriptase are included) and 2×TS miRNA Reaction Mix are supplied to efficiently add Poly(A) tails and synthesize first-strand cDNA. PerfectStartTM Green qPCR SuperMix is used for miRNA quantification.


• Optimal ratio of Poly(A) polymerase to reverse transcriptase and optimized reaction buffer, so as to ensure high transcription efficiency of miRNA.

• One-step completion of Poly(A) tail addition and first-strand cDNA synthesis in a single tube.

• High amplification efficiency, specificity and sensitivity ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.

• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).

Multiple copy and low copy gene detection.

at -20°C  in dark for two years

Dry ice (-70 ℃)

Product Contents

TransScript® miRNA RT Enzyme Mix20 μl
2×TS miRNA Reaction Mix200 μl
Universal miRNA qPCR Primer (10 μM)200 μl
PerfectStartTM Green qPCR SuperMix5×1 ml
Passive Reference Dye (50×)200 μl
RNase-free Water1 ml


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