SARS-CoV-2 Detection
  • DV101.jpg
DV101.jpg

SARS-CoV-2 Nucleic Acid Detection Kit

(Multiplex Real Time RT-PCR )


Product Details

Description

This real-time RT-PCR kit is intended for detection of ORF1ab and N genes from SARS-CoV-2 and for in vitro auxiliary diagnosis or emergency use of diagnosis of SARS-CoV-2 virus infection. The kit has a set of negative control, positive control and internal control. It has received CE-IVD mark. 


Highlights

• High sensitivity: Limit of detection (LoD) of 0.5 copies/μl. 

• High specificity: Proprietary Triple Antibody Blocking-based Hot Start Technology. No cross reactivity with HCoV-HKU1, HCoV-229E, HCoV-OC43 and HCoV-NL63.

• Fast workflow: Less than 60 min.

• Triplex RT-qPCR: Dual target assay for ORF1ab and N genes and an internal control gene.

• Minimized contamination : dUTP/ UNG used.


Instruments

Validated Instruments in-house: ABI 7500 and StepOnePlus Real-Time PCR instrument

Instruments used by customers with revised interpretation for test results: ABI QuantStudio 3, Bio-Rad CFX96.

Please contact our Technical Support team (trans@transgen.com.cn) for other instruments.


Sample Types

Pharyngeal swab or bronchoalveolar lavage specimens


Clinical Applications

Up to 600,000 reactions of real time RT-PCR for SARS-CoV-2 detection have been successfully applied in clinical diagnosis with our key components.


Storage

Store the kit at -20±5℃ away from light for 12 months.


Shipping

Ship the kit at low temperature. Dry ice should be used for long-distance shipping. Avoid repeated freeze-thaw cycles (freeze-thaw cycles should be fewer than 10).


Product Contents


ComponentMain Constituents

Specifications and 

Quantity(48 tests)

Specifications and 

Quantity(200 tests)

SARS-CoV-2 PCR Reaction MixReaction buffer, dNTPs, etc.720 μl×1 tube1000 μl×3 tubes
SARS-CoV-2 PCR Enzyme Mix

Reverse transcriptase, RNase inhibitor, 

Taq DNA polymerase, uracil-DNA 

glycosylase (UDG)

48 μl×1 tube200 μl×1 tube
SARS-CoV-2 PCR Primer/Probe Mix

Primers and probes for ORF1ab, N genes 

and the internal control-RNase P gene 

(RP)

192 μl×1 tube800 μl×1 tube
SARS-CoV-2 Positive Control

In vitro transcribed RNA for ORF1abN  

genes and the internal control-RP gene

50 μl×1 tube200 μl×1 tube
SARS-CoV-2 Negative ControlRNase-free Water50 μl×1 tube200 μl×1 tube


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