GelStain Blue is a sensitive, non-mutagenic and safer to the environment green fluorescent DNA gel dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. The sensitivity of GelStain Blue is much higher than GelStain and EB, and destaining is not required.
Highlights
• Nontoxic: Unique lipophilic and macromolecular propertie make it incapable of penetrating cell membranes. Ames test also shows the mutagenicity of GelStain Blue is far less than that of EB, and readily biodegradable, non-carcinogenic.
• Highly sensitive: Suitable for staining fragments of different sizes in electrophoresis gel;
• Highly stable: Suitable for using microwave or other heating methods to prepare agarose gel; extremely stable at room temperature under acidic or alkaline conditions; highly resistant to light.
• High signal-to-noise ratio: Strong fluorescence signal of the sample with low background signal.
• Simple operation: Similar to ethidium bromide, the dye does not degrade during the process of preparing gel or electrophoresis. It only takes 30 minutes for staining after electrophoresis, and the fragment can be visualized by a visible light-transilluminator directly without detaining or washing.
• A broad range of applications: Applicable for precast protocol (add dye during gel preparation) and post-stain protocol (submerge the gel in the staining solution); suitable for agarose gels and polyacrylamide gels electrophoresis; suitable for staining dsDNA, ssDNA or RNA.
• Existing imaging system: GelStain Blue has an optimal excitation in the UV region around 474 nm. It is recommended to try blue light for excitation observation. Standard UV-transilluminator used for EB staining observation can also be used.
Concentration
10000×
Storage
at room temperature protected from light for two years.
Shipping
at room temperature
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
