pEASY Cloning Vectors
  • CU201.jpg
CU201.jpg

pEASY®-Basic Seamless Cloning and Assembly Kit


Product Details

This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments.


• Fast: 15 minutes

• Simple: No restriction enzyme digestions.

• High efficiency: > 95% cloning efficiency.

• Seamless: No extra sequences introduced.


Storage

Trans1-T1 Phage Resistant Chemically Competent Cell at -70°C for six months; others at -20°C


Shipping

Dry ice (-70℃)

Product Contents


ComponentCU201-02CU201-03
2× Basic Assembly Mix100 μl3×100 μl
Linearized pUC19 Control Vector (10 ng/μl)6 μl18 μl
Control Insert (1 kb,20 ng/μl)6 μl18 μl
Trans1-T1 Phage Resistant Chemically Competent Cell10×100 μl30×100 μl


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