PCR Enzymes
  • AP221-01.JPG
AP221-01.JPG

TransStart® FastPfu DNA Polymerase


Product Details

TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.

• Extension rate is about 2-4 kb/min.
TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

Applications
• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAP221-01/11AP221-02/12AP221-03/13
TransStart® FastPfu DNA Polymerase250 U×1500 U×1500 U×6
TransStart® FastPfu Buffer1.2 ml×11.2 ml ×21.2 ml ×12
2.5 mM dNTPs- / 500 μl×1- / 1 ml ×1- / 1 ml ×6
50 mM MgSO4200 μl×1400 μl ×11 ml ×1
PCR Stimulant200 μl×1400 μl×11 ml ×1
6×DNA Loading Buffer200 μl×11 ml ×11 ml ×2


Citations

Zhang F , Tang W , Hedtke B , et al. Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing[J]. Proceedings of the National Academy of Sciences, 2014, 111(5):2023-2028.

Liang X , Dong X , Moerman D , et al. Sarcomeres Pattern Proprioceptive Sensory Dendritic Endings through UNC-52/Perlecan in C.elegans[J]. Developmental Cell, 2015, 33(4):388-400.

Huang X S , Wang W , Zhang Q , et al. A Basic Helix-Loop-Helix Transcription Factor, PtrbHLH, of Poncirus trifoliata Confers Cold Tolerance and Modulates Peroxidase-Mediated Scavenging of Hydrogen Peroxide[J]. PLANT PHYSIOLOGY, 2013, 162(2):1178-1194.

Ma Y , Li B , Wang C , et al. 5-HTTLPR Polymorphism Modulates Neural Mechanisms of Negative Self-Reflection[J]. Cerebral Cortex, 2014, 24(9):2421-2429. Cao Y X , Xiao W H , Liu D , et al. Biosynthesis of odd-chain fatty alcohols in Escherichia coli[J]. Metabolic Engineering, 2015, 29:113-123.

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