Cloning Competent Cells
  • CD411-02.JPG
CD411-02.JPG

Trans2-Blue Chemically Competent Cell


Product Details

Trans2-Blue Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to tetracycline (TetR) and chloramphenicol (CamR).

Genotype
TetRΔ(mcrA)183Hte[F′{proAB lacIq lacZΔM15Tn10(TetR)AmyCamR}]Δ(mcrCB-hsdSMR-mrr)173 endA1supE44 thi-1 recA1 gyrA96 relA1

Features
• High transformation efficiency (>1×108cfu/μg DNA).
• Suitable for larger plasmids and recombinant products.
• Reduced preference for plasmid size, suitable for library construction.
• Used in Blue/White selection.

Storage
at -70°C for six months. Do not store in liquid nitrogen

Shipping
Dry ice (-70℃)

Citations

Fang D , Xu G , Hu Y , et al. Identification of Genes Directly Involved in Shell Formation and Their Functions in Pearl Oyster, Pinctada fucata[J]. PLOS ONE, 2011, 6(7):e21860.

Jiang X Z , Pei Y X , Lei W , et al. Characterization of an insect heterodimeric voltage-gated sodium channel with unique alternative splicing mode[J]. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2017, 203:149-158.


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