pEASY Cloning Vectors

pEASY® -Blunt Zero Cloning Kit


Product Details

pEASY®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white selection is not required.

•5 minutes fast ligation of Pfu-amplified PCR products.
•High cloning efficiency. Positive clones up to 100%.
•No blue/white selection needed.
•Suitable for larger fragment cloning.
•KAN and AMP resistance genes for selection.
•M13 forward primer and M13 reverse primer for sequencing.
•T3 promoter and T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months

Shipping
Dry ice (-70℃)

Product Contents


ComponentCB501-01 (20 rxns)CB501-02 (60 rxns)
pEASY ®-Blunt Zero Cloning Vector (10 ng/μl)20 μl3×20 μl
Control Template (5 ng/μl)5 μl5 μl
Control Primers (10 μM)5 μl5 μl
M13 Forward Primer (10 μM)50 μl150 μl
M13 Reverse Primer (10 μM)50 μl150 μl
Trans1-T1 Phage Resistant Chemically Competent Cells10×100 μl30×100 μl


Citations

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He W , Huang T , Tang Y , et al. Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB[J]. Scientific Reports, 2015, 5(1):12368.

Hongyan W , Xiaoli T , Honglei W , et al. Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress[J]. Frontiers in Plant Science, 2015, 29(6):792.

Gao J , Wang G , Ma S , et al. CRISPR/Cas9-mediated targeted mutagenesis inNicotiana tabacum[J]. Plant Molecular Biology, 2015, 87(1-2):99-110.

Zhang X , He L , Zhang F , et al. The Different Potential of Sponge Bacterial Symbionts in N2 Release Indicated by the Phylogenetic Diversity and Abundance Analyses of Denitrification Genes, nirK and nosZ[J]. PLOS ONE, 2013, 10, 8(6):e65142.


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