pEASY®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white selection is not required.
•5 minutes fast ligation of Pfu-amplified PCR products.
•High cloning efficiency. Positive clones up to 100%.
•No blue/white selection needed.
•Suitable for larger fragment cloning.
•KAN and AMP resistance genes for selection.
•M13 forward primer and M13 reverse primer for sequencing.
•T3 promoter and T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months
Shipping
Dry ice (-70℃)
Gao F , Zhao J , Wang X , et al. NdhV Is a Subunit of NADPH Dehydrogenase Essential for Cyclic Electron Transport in Synechocystis sp. Strain PCC 6803[J]. Plant Physiology, 2016, 170(2):752.
He W , Huang T , Tang Y , et al. Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB[J]. Scientific Reports, 2015, 5(1):12368.
Hongyan W , Xiaoli T , Honglei W , et al. Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress[J]. Frontiers in Plant Science, 2015, 29(6):792.
Gao J , Wang G , Ma S , et al. CRISPR/Cas9-mediated targeted mutagenesis inNicotiana tabacum[J]. Plant Molecular Biology, 2015, 87(1-2):99-110.
Zhang X , He L , Zhang F , et al. The Different Potential of Sponge Bacterial Symbionts in N2 Release Indicated by the Phylogenetic Diversity and Abundance Analyses of Denitrification Genes, nirK and nosZ[J]. PLOS ONE, 2013, 10, 8(6):e65142.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
