RT-PCR
  • AH341-01.JPG
AH341-01.JPG

TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )


Product Details

The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR.

• Simultaneous genomic DNA removal and cDNA synthesis.
• The optimal ratio of Oligo(dT)20 Primer to random primer(N9) for qPCR ready cDNA.
• qPCR ready cDNA in 15 minutes.
• cDNA up to 250 bp.

Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAH341-01 (50 rxns )
TransScript® II All-in-One SuperMix for qPCR200 μl
TransScript® II All-in-One No-RT Control SuperMix for qPCR20 μl
gDNA Remover50 μl
RNase-free Water1 ml


Citations

Cui X . Exploring the formation and recognition of an important G-quadruplex in a HIF1α promoter and its transcriptional inhibition by a benzo[c]phenanthridine derivative.[J]. Journal of the American Chemical Society, 2014, 136(6):2583.

Gu Q , Chen Z , Yu X , et al. Melatonin confers plant tolerance against cadmium stress via the decrease of cadmium accumulation and reestablishment of microRNA-mediated redox homeostasis[J]. Plant Science, 2017, 261:28-37.

Huang X , Huang B , Chen J , et al. Cellular responses of the dinoflagellate\r, Prorocentrum donghaiense\r, Lu to phosphate limitation and chronological ageing[J]. Journal of Plankton Research, 2016, 38(1):83-93.

Guo J , Zu G , Zhou T , et al. Clinicopathological significance of orphan nuclear receptor Nurr1 expression in gastric cancer[J]. Clinical and Translational Oncology, 2015, 17(10):788-794.

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