DNA/RNA extraction is usually the first step required for most molecular biology investigations in plant research. Plants present some unique challenges compared to animal tissues in DNA/RNA extraction. For example, the tough cell wall of many plant tissues often requires time-consuming enzymatic or mechanical techniques to break apart. In addition, other problems may be encountered including the presence of large quantities of polysaccharides; high levels of RNases; various different kinds of phenolics, including tannins; low concentrations of nucleic acids (high water content); tissue, such as lignin (wood), that is difficult to break up; and so on. To overcome these problems and difficulties, TransGen provides both silica-based spin column method and modified CTAB method tailored for plant DNA/RNA extraction. Furthermore, soil DNA extraction reagent based on magnetic bead method is also available.
Plant DNA Extraction in Plant Research-Silica-based Spin Column Method
• EasyPure® Plant Genomic DNA Kit (Cat. No. EE111)
√ Fast workflow & high yield (up to 15 μg/100 mg plant tissue).
√ Complete removal of pigment, polysaccharides, phenols and other impurities to improve binding efficiency of DNA.
Application data from the publication: Zhang Y, Li Z X, Yu X D, et al. Molecular characterization of two isoforms of a farnesyl pyrophosphate synthase gene in wheat and their roles in sesquiterpene synthesis and inducible defence against aphid infestation[J]. New Phytologist, 2015, 206(3): 1101-1115.
Fig. 1 Genomic structure of Tafps detected by GSDS analysis after the genomic DNA was extracted from wheat using
EasyPure® Plant Genomic DNA Kit (Cat. No. EE111).
Plant DNA Extraction in Plant Research- Modified CTAB Method
• PlantZol (Cat. No. EE141)
√ Suitable for samples rich in polysaccharides and polyphenols.
Application data from the publication: Zhang L, Lan Q, Han S, et al. A GH3-like gene, LaGH3, isolated from hybrid larch (Larix leptolepis× Larix olgensis) is regulated by auxin and abscisic acid during somatic embryogenesis[J]. Trees, 2019, 33(6): 1723-1732.
Fig. 2 Structure of the promoter region of the LaGH3 gene obtained by bioinformatics analysis after genomic DNA extraction
from L. leptolepis × L. olgensis using PlantZol (Cat. No. EE141), PCR and cloning of the promoter region.
Plant RNA Extraction in Plant Research-Silica-based Spin Column Method
• EasyPure® Plant RNA Kit (Cat. No. ER301)
√ High purity with no protein/DNA contamination.
Application data from the publication: Liu Q, Deng S, Liu B, et al. A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease[J]. Nature communications, 2020, 11(1): 1-14.
Fig. 3 The transgenic plants which express exogenous ZmGDIα gene were validated by RT-PCR experiments after
total RNA was extracted from maize leaf using EasyPure® Plant RNA Kit (Cat. No. ER301).
Plant RNA Extraction in Plant Research-Modified CTAB Method
• TransZol Plant (Cat. No. ET121)
√ Suitable for samples rich in polysaccharides and polyphenols, such as champignon, banana fruit, mango fruit, potato tubers, carrot, Tiger Piran leaf, etc.
Application data from the publication: Tang G, Xing S, Wang S, et al. Regulation of cysteine residues in LsrB proteins from Sinorhizobium meliloti under free‐living and symbiotic oxidative stress[J]. Environmental microbiology, 2017, 19(12): 5130-5145.
Fig. 4 The expression of lsrB, lrp3, katA and gshA in alfalfa nodules detected by qRT-PCR analysis after root nodules from
20 two-week-old alfalfa plants were treated by RNA extraction using TransZol Plant (Cat. No. ET121).
Soil DNA Extraction in Plant Research-Modified CTAB Method
• MagicPure® Stool and Soil Genomic DNA Kit (Cat. No. EC801)
√ Simple and fast
√ High yield and purity
Riverside sand soil 1 Riverside sand soil 2 Riverside sand soil 3 Farmland soil sample
Fig 5 Agarose gel electrophoresis results for genomic DNA extracted from 250 mg of different types of soil samples using
MagicPure® Stool and Soil Genomic DNA Kit (Cat. No. EC801) and the product from Company T, respectively.
References
Tamari F, Hinkley C S. Extraction of DNA from plant tissue: review and protocols[M]//Sample preparation techniques for soil, plant, and animal samples. Humana Press, New York, NY, 2016: 245-263.
Yockteng R, Almeida A M R, Yee S, et al. A method for extracting high‐quality RNA from diverse plants for next‐generation sequencing and gene expression analyses[J]. Applications in plant sciences, 2013, 1(12): 1300070.
Related Pages for DNA/RNA Extraction Reagents in Plant Research
• Integrated Plant Research Reagent Collection
