EasyPure® Plant Genomic DNA Kit provides a simple and convenient way to isolate high quality plant genomic DNA from plant tissues (up to 100 mg). Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent. Proteins, polysaccharides, and cell debris are eliminated by precipitation. DNA is bound to silica-based column.
• DNA yield up to 15 μg.
• Complete removal of pigment, polysaccharides and other impurity.
• DNA range of 20-50 kb.
• Column based purification, no organic extraction or ethanol precipitation.
Applications
PCR, restriction enzyme digestion and Southern blotting
Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) for one year
Shipping
RNase A on dry ice (-70 °C); Others at room temperature
Zhang Y , Li Z X , Yu X D , et al. Molecular characterization of two isoforms of a farnesyl pyrophosphate synthase gene in wheat and their roles in sesquiterpene synthesis and inducible defence against aphid infestation[J]. New Phytologist, 2015, 206(3):1101-1115.
Ju Z , Cao D , Gao C , et al. A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants[J]. Plant Physiology, 2017, 173(4):1940-1952.
Liu P , Qi M , Wang Y , et al. Arabidopsis RAN1 Mediates Seed Development through Its Parental Ratio by Affecting the Onset of Endosperm Cellularization[J]. Molecular Plant, 2014, 7(8):1316-1328.
Chen K , Han H , Luo Z , et al. A practicable detection system for genetically modified rice by SERS-barcoded nanosensors[J]. Biosensors and Bioelectronics, 2012, 34(1):118-124.
Bai N , Wang S , Abuduaini R , et al. Rhamnolipid-aided biodegradation of carbendazim by, Rhodococcus, sp. D-1: Characteristics, products, and phytotoxicity[J]. Science of The Total Environment, 2017, 590-591:343-351.
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
Instruments
SARS-CoV-2 Detection
Residual DNA Detection
Animal Disease
