Genomic DNA Purification
  • EE111-01.JPG
EE111-01.JPG

EasyPure® Plant Genomic DNA Kit


Product Details

EasyPure® Plant Genomic DNA Kit provides a simple and convenient way to isolate high quality plant genomic DNA from plant tissues (up to 100 mg). Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent. Proteins, polysaccharides, and cell debris are eliminated by precipitation. DNA is bound to silica-based column.

• DNA yield up to 15 μg.
• Complete removal of pigment, polysaccharides and other impurity.
• DNA range of 20-50 kb.
• Column based purification, no organic extraction or ethanol precipitation.

Applications
PCR, restriction enzyme digestion and Southern blotting

Storage
RNase A at -20°C for one year; others at room temperature (15-25°C) for one year

Shipping
RNase A  on dry ice (-70 °C); Others at room temperature

Product Contents


ComponentEE111-01 (50 rxns)EE111-02 (200 rxns)EE111-11 (50 rxns)EE111-12 (200 rxns)
Resuspension Buffer1 (RB1)15 ml60 ml15 ml60 ml
Precipitation Buffer1 (PB1)6 ml25 ml6 ml25 ml
Binding Buffer1 (BB1)8 ml32 ml8 ml32 ml
Clean Buffer 1 (CB1)30 ml110 ml30 ml110 ml
Wash Buffer 1 (WB1)12 ml2×22 ml12 ml2×22 ml
Elution Buffer (EB)25 ml80 ml25 ml80 ml
RNase A (10 mg/ml)800 μl4×800 μl00
Genomic Spin Column with Collection Tubes50 each200 each50 each200 each


Citations

Zhang Y , Li Z X , Yu X D , et al. Molecular characterization of two isoforms of a farnesyl pyrophosphate synthase gene in wheat and their roles in sesquiterpene synthesis and inducible defence against aphid infestation[J]. New Phytologist, 2015, 206(3):1101-1115.

Ju Z , Cao D , Gao C , et al. A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants[J]. Plant Physiology, 2017, 173(4):1940-1952.

Liu P , Qi M , Wang Y , et al. Arabidopsis RAN1 Mediates Seed Development through Its Parental Ratio by Affecting the Onset of Endosperm Cellularization[J]. Molecular Plant, 2014, 7(8):1316-1328.

Chen K , Han H , Luo Z , et al. A practicable detection system for genetically modified rice by SERS-barcoded nanosensors[J]. Biosensors and Bioelectronics, 2012, 34(1):118-124.

Bai N , Wang S , Abuduaini R , et al. Rhamnolipid-aided biodegradation of carbendazim by, Rhodococcus, sp. D-1: Characteristics, products, and phytotoxicity[J]. Science of The Total Environment, 2017, 590-591:343-351.


Message