• AT341-01.JPG

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )

Product Details

The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR.

• Simultaneous genomic DNA removal and cDNA synthesis.
• The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
• qPCR ready cDNA in 15 minutes.
• cDNA up to 250 bp.

Multiple copy and low copy gene detection

at -20 ℃ for two years

Dry ice (-70 ℃)

Product Contents

ComponentAT341-01 (50 rxns )AT341-02 (100 rxns )AT341-03 (500 rxns )
TransScript® All-in-One SuperMix for qPCR200 μl400 μl5×400 μl
TransScript® All-in-One No-RT Control SuperMix for qPCR20 μl40 μl200 μl
gDNA Remover50 μl100 μl5×100 μl
RNase-free Water1 ml2×1 ml2×5 ml


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