pEASY® -Uni Seamless Cloning and Assembly Kit-CU101

   Product Name  

pEASY®-Uni Seamless Cloning and Assembly Kit (CU101) 

  High-Impact Factor Literature Collection  

•Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022.(IF 63.71)

•Shi J, Mei C, Ge F, et al. Resistance to Striga parasitism through reduction of strigolactone exudation[J]. Cell, 2025.(IF 42.50)

•Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 38.07)

•Wang H, Yang J, Cai Y, et al. Macrophages suppress cardiac reprogramming of fibroblasts in vivo via IFN-mediated intercellular self-stimulating circuit[J]. Protein & Cell, 2024.(IF 21.10)

•Xu J, Liang Y, Li N, et al. Clathrin-associated carriers enable recycling through a kiss-and-run mechanism[J]. Nature Cell Biology, 2024.(IF 17.30)

•Li B, Zhu L, Lu C, et al. circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor  immunity[J]. Nature communications, 2021.(IF 14.92)

•Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research,  2022.(IF 14.90)

•Wang J, An Z, Wu Z, et al. Spatial organization of PI3K-PI (3, 4, 5) P3-AKT signaling by focal adhesions[J]. Molecular Cell, 2024.(IF 14.50)

•Liu S, Fan L, Liu Z, et al. A Pd1–Ps–P1 feedback loop controls pubescence density in soybean[J]. Molecular plant, 2020.(IF 12.08)

•Jin Q, Yang X, Gou S, et al. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and  switchable gene expression[J]. Science China Life Sciences, 2022.(IF 10.37)

•Mu S, Chen H, Li Q, et al. Enhancing prime editor flexibility with coiled-coil heterodimers[J]. Genome Biology, 2024.(IF 10.10)

•Du G, Xiong L, Li X, et al. Peroxisome elevation induces stem cell differentiation and intestinal epithelial repair[J]. Developmental cell, 2020. (IF 10.09)

•Tang Y, Gao C C, Gao Y, et al. OsNSUN2-mediated 5-methylcytosine mRNA modification enhances rice adaptation to high  temperature[J]. Developmental cell, 2020.(IF 10.09)

•Li J, Bai Y, Liu Y, et al. Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems[J]. Cell Reports, 2023.(IF 8.80)

•Que Y, Shu X, Wang L, et al. Inactivation of SERCA2 Cys674 accelerates aortic aneurysms by suppressing PPARγ[J]. British Journal of  Pharmacology, 2021.(IF 8.74)

•Wang Y, Dong H, Qu Y, et al. Circabidian rhythm of sex pheromone reception in a scarab beetle[J]. Current Biology, 2024.(IF 8.10)

•Sun S, Liu D, Luo W, et al. Domestication-selected COG4-OsbZIP23 module regulates chilling tolerance in rice[J]. Cell Reports, 2024.(IF 7.50)

•Du J, Huang Z, Li Y, et al. Copper exerts cytotoxicity through inhibition of iron-sulfur cluster biogenesis on ISCA1/ISCA2/ISCU assembly  proteins[J]. Free Radical Biology and Medicine, 2023.(IF 7.40)

•Zhu L, Li B, Li R, et al. METTL3 suppresses pancreatic ductal adenocarcinoma progression through activating endogenous dsRNA-induced  anti-tumor immunity[J]. Cellular Oncology, 2023.(IF 6.60)

•Song N, Chen L, Ren X, et al. N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian  cells[J]. PLoS Pathogens, 2021.(IF 6.20)

  Features 

• High Cloning Efficiency: High positive rate, abundant colonies.

• Multi-Fragment Assembly: Enables seamless assembly of up to 15 fragments.

• Rapid Reaction: Completes the recombination process in just 5-15 minutes.

• Large Capacity Assembly: Successfully constructs plasmids up to 31.8 kb (vector + insert).

• Long Vector Compatibility: Efficiently assembles long vectors up to 14 kb.

• Broad Compatibility: Supports highly efficient assembly of single or multiple fragments, even at low concentrations (e.g., 0.003 pmol).

• Online Primer Design Tool: Provides an intelligent online software for rapid generation of high-specificity primers.

  Data Presentation  

High Assembly Efficiency

High Assembly Efficiency

Successful Assembly with 14 kb Long Vector

Successful Assembly with 14 kb Long Vector

Successful Low-Concentration Multi-Fragment Assembly ((Fragment input amount is 1/10 of routine input (0.003 pmol))

Successful Low-Concentration Multi-Fragment Assembly

  Product Information  

Product Information

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