TransScript All-in-One First-Strand cDNA Synthesis SuperMix (AT341)
Product Name
Patent
Patent Number：ZL 201710031139.6
High-Impact Factor Literature Collection
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Features
• All-in-One SuperMix Format: Simply add gDNA Remover, template RNA, and water. This allows for simultaneous cDNA synthesis and genomic DNA removal.
• Optimized Reaction System: Features an optimized blend of Oligo(dT)18 Primer and Random Primer (N9), along with the SuperMix composition. This ensures consistent reverse transcription efficiency across a wide range of RNA concentrations and high efficiency for short cDNA fragment synthesis.
• Fast Procedure: The reverse transcription process requires only 15 minutes.
• High Compatibility: Demonstrates excellent compatibility with mainstream qPCR reagents.
• Application Note: The resulting cDNA is suitable for qPCR only and is not recommended for conventional PCR.
Data Presentation
High Amplification Efficiency
RT was performed with 10 fold diluted RNA (1 μg to 100 pg) from different species as template, using reagents from TransGen and Company V. The resulting cDNA was used as template for qPCR using dye-based qPCR reagent (AQ601) from TransGen. The result showed that TransGen product exhibited high amplification efficiency.
Product Information
