TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix -AT311
Product Name
TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311)
Patent
Patent Number：ZL201710031139.6
High-Impact Factor Literature Collection
•Gong Q, Wang Y, He L, et al. Molecular basis of methyl-salicylate-mediated plant airborne defence[J]. Nature, 2023.(IF 64.80)
•Guan J, Wang G, Wang J, et al. Chemical reprogramming of human somatic cells to pluripotent stem cells[J]. Nature, 2022.(IF 49.00)
•Wang J Z, Chen T Y, Zhang Z D, et al. Remodelling autoactive NLRs for broad-spectrum immunity in plants [J]. Nature, 2025.(IF 48.50)
•Wang S, Du Y, Zhang B, et al. Transplantation of chemically induced pluripotent stem-cell-derived islets under abdominal anterior rectus sheath in a type 1 diabetes patient[J]. Cell, 2024.(IF 45.50)
•Zhao P Z, Yang H, Sun J Y, et al. Targeted MYC2 stabilization confers citrus Huanglongbing resistance [J]. Science, 2025.(IF 44.70)
•Chen J, Ou Y, Luo R, et al. SAR1B senses leucine levels to regulate mTORC1 signalling[J]. Nature, 2021.(IF 42.77)
•Chen J, Ou Y, Yang Y, et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018.(IF 40.13)
•Zhao K, Xue H, Li G, et al. Pangenome analysis reveals structural variation associated with seed size and weight traits in peanut[J]. Nature genetics, 2025.(IF 31.80)
•Liu S, Liu C, Lv X, et al. The chemokine CCL1 triggers an AMFR-SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis[J]. Immunity, 2021.(IF 31.74)
•Huang L, Wei M, Li H, et al. GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis[J]. Molecular Cancer, 2025.(IF 27.70)
•Fan H, Quan S, Ye Q, et al. A molecular framework underlying low-nitrogen-induced early leaf senescence in Arabidopsis thaliana[J]. Molecular Plant, 2023.(IF 27.50)
•Zhu J, Zhong X, He H, et al. Generation of human expandable limb-bud-like progenitors via chemically induced dedifferentiation[J]. Cell Stem Cell, 2024.(IF 19.80)
•Wang R, Xue Y, Fan J, et al. A systems genetics approach reveals PbrNSC as a regulator of lignin and cellulose biosynthesis in stone cells of pear fruit[J]. Genome Biology, 2021.(IF 17.90)
•Zhang Y, Chen W, Wu D, et al. Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli[J]. Nucleic acids research, 2022.(IF 16.97)
•Wang Z, Sheng C, Kan G, et al. RNAi Screening Identifies that TEX10 Promotes the Proliferation of Colorectal Cancer Cells by Increasing NF - κB Activation[J]. Advanced Science, 2020.(IF 15.84)
•Zhao M, Wang B, Zhang C, et al. The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson's disease[J]. Cell Death & Differentiation, 2021.(IF 15.82)
•Liu J C, Li L, Yan H C, et al. Identification of oxidative stress–related Xdh gene as a di (2 - ethylhexyl) phthalate (DEHP) target and the use of melatonin to alleviate the DEHP - induced impairments in newborn mouse ovaries[J]. Journal of pineal research, 2019.(IF 15.22)
•Zhang C, Wang Y, Zhen Z, et al. mTORC1 Mediates Biphasic Mechano - Response to Orchestrate Adhesion - Dependent Cell Growth and Anoikis Resistance[J]. Advanced Science, 2023.(IF 15.10)
•Wang Y, Wang Z, Chen W, et al. A KDPG sensor RccR governs Pseudomonas aeruginosa carbon metabolism and aminoglycoside antibiotic tolerance[J]. Nucleic Acids Research, 2023.(IF 14.90)
Features
• Simultaneously perform reverse transcription and genomic DNA removal within the same reaction system. The operation is simple and reduces the risk of contamination.
• For qPCR applications: reverse transcription time is 15 minutes; for PCR applications: reverse transcription time is 30 minutes.
• After the reaction, thermally inactivate both the Reverse Transcriptase/RI and gDNA Remover at the same time.
• Can synthesize cDNA fragments up to 12 kb in length.
Data Presentation
Comparsion with Competitive Products
RT-PCR was performed with 100 ng of human total RNA (1), 100 ng of human total RNA and 200 ng gDNA (2), 200 ng gDNA (3), using reagents from TransGen, Company A and Company B.
Product Information
