RT-PCR
  • AT311-02.JPG
AT311-02.JPG

TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix


Product Details

Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 12 kb.

Applications
Multiple copy and low copy gene detection

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAT311-02 (50 rxns)AT311-03 (100 rxns)AT311-04 (500 rxns)
TransScript® RT/RI Enzyme Mix50 μl100 μl5×100 μl
gDNA Remover50 μl100 μl5×100 μl
2×TS Reaction Mix500 μl1 ml5×1 ml
Random Primer(N9) (0.1 μg/μl)50 μl100 μl5×100 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl)50 μl100 μl5×100 μl
RNase-free Water500 μl1 ml5 ml


Citations

Chen J , Ou Y , Yang Y , et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018,557(7706):585-589.

Yang Y , Liu B , Xu J , et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency[J]. Cell, 2017, 169(2):243-257.e25.

Wu D , Qi T , Li W X , et al. Viral effector protein manipulates host hormone signaling to attract insect vectors[J]. Cell Research, 2017, 27(3):402-415.

Shao X , Liu Y , Yu Q , et al. Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b[J]. Cell Research, 2016,26(5):593-612.

Ma D , Hu Y , Yang C , et al. Genetic basis for glandular trichome formation in cotton[J]. Nature Communications, 2016, 7:10456.

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