AQ7 Series Probe-Based qPCR Solutions – Precision for Every Testing Need
Quantitative Real-time PCR (qPCR) is a vital method for studying gene expression levels. It utilizes changes in fluorescence signals to monitor the amount of amplified product in real-time during each PCR cycle, enabling quantitative analysis of the initial template through the relationship between Ct values and standard curves. Currently, qPCR is widely applied across various fields of molecular biology, including: Diagnosis of human infectious diseases, Detection of pathogenic genes in animals, Inspection and quarantine of livestock and poultry products, Quality control and identification of biological products,etc.
The two most commonly used qPCR detection methods are the SYBR Green dye-based method and the fluorescent probe-based method. The non-specific SYBR Green dye method is more cost-effective but may yield false-positive results due to interference from non-specific PCR amplification products. In contrast, the probe-based method uses fluorescent probes to ensure synchronous accumulation of fluorescence signals with PCR product formation. This approach offers higher sensitivity, superior specificity, and simpler operation, making it the most widely used technique in clinical diagnostics today.
With 19 years of expertise in enzyme engineering and product development, TransGen Biotech has been consistently delivering high-quality, cost-effective solutions to IVD manufacturers, scientific institutions, and third-party testing organizations. Our products have been successfully applied in: Gene expression analysis, Pathogen detection, Genotyping, Copy number variation analysis, empowering researchers to achieve groundbreaking scientific advancements.
Basic
The PerfectStart® series probe-based qPCR reagents are high-performance premixed solutions specifically designed for precise and sensitive qPCR detection. The core enzyme is the PerfectStart® series hot-start DNA polymerase, a genetically engineered Taq DNA polymerase that utilizes antibody-mediated inactivation to effectively block polymerase activity at low temperatures, preventing non-specific amplification. When combined with an optimized reaction buffer, this system significantly enhances template affinity and qPCR amplification performance. It delivers improved sensitivity for low-copy targets and demonstrates excellent tolerance to impurities in nucleic acids (including PCR inhibitors), making it suitable for a wide range of detection scenarios.
PerfectStart® II Probe qPCR SuperMix (AQ711)
PerfectStart® II Probe qPCR SuperMix UDG (AQ712)
Features
• Blocking by 3 antibodies, with high specificity, high sensitivity, and high amplification efficiency.
• A specially optimized qPCR reaction buffer for enhanced sensitivity and specificity.
• Comes with a Passive Reference Dye (to minimize well-to-well differences) compatible with different qPCR instruments, guaranteeing reliable results.
• Wide range of applications: Successfully used for detecting African swine fever virus, Pseudorabies virus, Vibrio harveyi, Enterocytozoon hepatopenaei, and Vibrio parahaemolyticus,etc.
• Excellent stability: Maintains robust amplification performance even after repeated freeze-thaw cycles or storage in premix, at room temperature, or at 37°C.
• dUTP/UDG system: Effectively prevents carry-over contamination caused by PCR products, ensuring accurate results.
Application
Suitable for probe-based qPCR detection of DNA from animal, plant, and microbial, demonstrating superior species compatibility.
High sensitivity and amplification efficiency
Using different concentrations of PRRSV cDNA (10 pg, 0.1 pg, 0.01 pg) and BVDV cDNA (100 pg, 1 pg, 0.1 pg) as templates, amplification was performed with products from TransGen, Company V, and Company T. The results demonstrated that TransGen's product achieved a sensitivity of 0.1 pg, with superior amplification efficiency compared to Company V and Company T.
Wide range of applications
TransGen's product successfully detected DNA from Vibrio harveyi, Enterocytozoon hepatopenaei (EHP), and Vibrio parahaemolyticus across serial 10-fold dilutions (107 to 104copies/mL)
PerfectStart® III Fast Probe qPCR SuperMix UDG (AQ722)
Features
• Antibody-blocking technology with high specificity, sensitivity, and amplification efficiency, demonstrating strong inhibitor resistance and broad species compatibility.
• A specially optimized qPCR reaction buffer for enhanced sensitivity and specificity.
• Ultra-fast detection: Detection can be completed in as little as 35 minutes.
• High amplification capability: Suitable for multiplex PCR applications.
• Excellent stability—no performance degradation after 25 freeze-thaw cycles, 1-week storage at 37℃ , or 15 days at room temperature.
• dUTP/UDG system: Effectively prevents carry-over contamination caused by PCR products, ensuring accurate results.
• Supports full premix formulation of primers and probes.
• With universal passive reference dye for signal calibration across wells.
Application
Suitable for complex sample detection, with validated applications in HPV, HBV, and animal pathogen testing.
Rapid amplification
Using serially diluted African swine fever virus (ASFV) genomic DNA (gDNA) as template, rapid qPCR was performed with TransGen's kit. Results demonstrated TransGen's ultra-fast amplification capability, requiring only 1-second extension time and completing the entire process in 35 minutes.
High GC tolerance
Using pseudorabies virus (PRV) gDNA (69% GC content) as template, qPCR was performed with TransGen and Company V kits. Results demonstrated TransGen's superior amplification performance for complex templates.
PerfectStart® IV Fast Probe qPCR SuperMix UDG (AQ732)
Features
• High specificity: Utilizes next-generation hot-start Taq DNA polymerase with advanced antibody-blocking technology.
• High inhibitor tolerance: Enables direct amplification from blood, tissue homogenates, swabs, and other complex samples.
• Fully premix-compatible: Supports complete primer/probe premixing.
• Excellent stability: Maintains consistent performance after 50 freeze-thaw cycles, 30-day storage at 37°C, or 30-day stress testing at 37°C.
• Ultra-fast PCR: Completes 45 amplification cycles in 35 minutes.
• dUTP/UDG system: Effectively prevents carry-over contamination caused by PCR products, ensuring accurate results.
Application
Demonstrates superior performance with swab, blood, and impurity-containing samples. Successfully applied to challenging detection scenarios including African swine fever (ASFV) in low-quality extracts, HPV, lung cancer, and respiratory bacterial pathogens.
High impurity tolerance
Using ASFV-positive tissue homogenate and healthy swine blood added with ASFV gDNA as templates separately, qPCR targeting the ASFV national standard gene was performed with TransGen and Company V kits. Results demonstrated TransGen's superior inhibitor resistance.
Excellent direct PCR performance
Using nucleic acid release reagent-treated oral/nasal swabs samples from healthy pigs, nucleic acid release reagent and water as diluents, ASFV gDNA was diluted to equal nucleic acid concentrations. Quadruplex qPCR with TransGen’s kit demonstrated reliable direct amplification from swab samples, proving compatibility with swab-based direct detection.
Extraction-Free qPCR
Extraction-Free qPCR is a reaction that enables direct amplification from crude samples without prior nucleic acid extraction. TransGen Biotech's extraction-free fluorescent quantitative PCR rapid detection products utilize highly robust enzymes and specially formulated buffers to perform nucleic acid amplification directly from lysed biological samples while ensuring result accuracy. This technology significantly reduces detection time, saves labor costs and experimental resources, and improves testing efficiency.
PerfectStart® V Fast Direct Probe qPCR SuperMix UDG (AQ742)
Features
• Simple workflow: Enables direct target gene detection without nucleic acid extraction or purification steps.
• Enhanced inhibitor resistance: Incorporates next-generation Taq DNA polymerase with improved template affinity and qPCR performance, demonstrating high tolerance for impurity-containing samples.
• Rapid amplification: Compatible with fast-cycling protocols, completing PCR in 35 minutes.
• dUTP/UDG system: Effectively prevents carry-over contamination caused by PCR products, ensuring accurate results.
Application
Extraction-free amplification for whole blood (anticoagulated), plasma, serum, and throat swab samples.
Using healthy swine whole blood of different concentration and water as templates (added with ASFV-positive nucleic acid ) for qPCR, the results showed that AQ742 performed almost identically in samples containing 0.5% whole blood compared to blood-free samples. Even under conditions with 10% whole blood, it remained capable of effective detection, demonstrating strong resistance to inhibition and suitability for direct amplification of blood samples.
Disease Diagnosis
Probe-based qPCR technology offers rapid development, high sensitivity, and excellent specificity, making it well-suited for: Rapid screening and diagnosis of emerging infectious diseases, detection of viral infections with asymptomatic or early-stage presentations, and differential diagnosis of pathogens causing similar clinical symptoms. Multiplex nucleic acid detection technology enables simultaneous detection of multiple targets with high throughput and cost efficiency, meeting the demands of large-scale screening and quantitative analysis. TransGen Biotech has launched a series of detection kits for HPV, HBV, and animal pathogens, delivering fast, multiplex, and precise diagnostic solutions.
PerfectStart® III Fast Probe qPCR SuperMix UDG (P) (AQ722-S1)
Application
Suitable for ultra-multiplex HPV detection
Using AQ722-S1 for multiplex HPV testing, the results demonstrated that AQ722-S1 successfully achieved detection at 20 copies/reaction, exhibiting high sensitivity and excellent specificity.
PerfectStart® III Fast Probe qPCR SuperMix UDG(B) (AQ722-S2)
Application
HBV detection
HBV virus was detected by AQ722-S2. The AQ722-S2 demonstrated reliable detection at 1 IU/reaction, with no false positives for healthy human gDNA, offering high sensitivity and superior specificity.
PerfectStart® IV Fast Probe qPCR SuperMix UDG(P) (AQ732-S1)
This product is a pre-mix reagent specially designed for ultra-multiplex detection of HPV using probe-based qPCR. It is supplied at a 2× concentration, simply add primers, probe and template to perform multiplex PCR reaction in one reaction well. This product contains PerfectStart® IV Taq hot-Start DNA polymerase (Genetically modified fourth generation Taq DNA polymerase), which significantly improves template affinity, qPCR amplification performance and sensitivity. In addition to amplifying extracted nucleic acids, this product facilitates direct amplication from crude lysates of cervical scrapings, eliminating the need for DNA purification. The dUTP/UDG in the SuperMix can function at room temperature to eliminate carry-over contamination caused by PCR products and aerosol to ensure the accuracy of results.
Application
Ultra-multiplex HPV detection directly from swab samples
PerfectStart® IV Fast Probe qPCR SuperMix UDG (AQ732-S2)
This product is a pre-mix reagent specially designed for animal disease detection using probe-based qPCR. It is supplied at a 2× concentration, simply add primers, probe and template to perform singleplex or multiplex PCR reaction in one reaction well. This product contains PerfectStart® IV Taq hot-start DNA polymerase (Genetically modified fourth generation Taq DNA polymerase), which significantly improves template affinity, qPCR amplification performance and detection sensitivity for inhibitor-containing or low-concentration samples. The dUTP/UDG in the SuperMix can function at room temperature to eliminate carry-over contamination caused by PCR products and aerosol to ensure the accuracy of results.
Application
Animal disease detection
Genotyping
Genotyping is the process of identifying genetic variations among individuals within a population. Single nucleotide polymorphisms (SNPs), or point mutations, are the most common type of genetic variation and account for a significant portion of phenotypic diversity among individuals. As third-generation genetic markers, SNPs can reveal differences in genetic material between individuals. Some SNP loci may also affect gene function, leading to phenotypic changes or even disease. Therefore, SNP detection is an essential component of genetic research and related fields. There are various technologies available for SNP detection, including PCR-based methods, sequencing, and microarrays. TransGen offers three methods for SNP detection: ARMS-qPCR, TaqMan SNP probe assay and high-resolution melt curve analysis.
PerfectStart® IV Multiplex Probe ARMS qPCR SuperMix UDG (AQ734)
Features
• High sensitivity and specificity: This product contains newly upgraded PerfectStart® IV hot-start Taq DNA polymerase, blocking by 3 antibodies, combined with the reaction buffer optimized for amplification-refractory mutation system (ARMS), enhancing both sensitivity and specificity.
• Easy to use: Supplied at a 2× concentration, simply add primers, probe and template to perform multiplex reaction.
• dUTP/UDG system: Effectively prevents carry-over contamination caused by PCR products, ensuring accurate results.
Application
Precision molecular diagnostics in oncology, like EGFR mutation detection in tumor targeted therapy
PerfectStart® V Probe SNP Genotyping qPCR SuperMix UDG (AQ744)
Features
• High specificity: The newly upgraded PerfectStart® IV hot-start Taq DNA polymerase, a next-generation genetically engineered Taq DNA polymerase, offers high specificity in genotyping detection across various sample types.
• Easy to use: Supplied at a 2× concentration, simply add primers, probe and template to perform multiplex reaction.
• dUTP/UDG system: Effectively prevents carry-over contamination caused by PCR products, ensuring accurate results.
Application
Widely used in large-scale SNP detection and gene expression analysis
The human MTHFR C677T polymorphism was detected using AQ744. AQ744 can effectively distinguish all three genotypes (CC, CT, TT) at the MTHFR C677T locus. This enables more efficient and accurate support for folate metabolism screening and anomaly management.
PerfectStart® V Multiplex Probe Melt qPCR Mix UDG (AQ745)
Features
• High uniformity and specificity: Utilizing specially processed PerfectStart® V hot-start Taq DNA polymerase, this system delivers excellent performance in melting curve peak clarity, uniformity, and specificity in ultra-multiplex amplification.
• Easy to use: Supplied at a 2× concentration, simply add primers, probe and template to perform multiplex reaction.
• dUTP/UDG system: Effectively prevents carry-over contamination caused by PCR products, ensuring accurate results.
Application
Pathogen detection and identification (such as HPV genotyping), gene mutation analysis, drug resistance gene detection, etc.
ALDH2 gene polymorphism detection
AQ745 enables rapid, efficient, and accurate genotyping of ALDH2, providing critical guidance for the timely treatment of angina with nitroglycerin, alcohol consumption recommendations, and assessing cancer risks.
Ultra-multiplex HPV genotyping
AQ745 achieves highly multiplexed genotyping of various HPV subtypes, offering precise diagnostic support for HPV infection detection.
Digital PCR
Digital PCR (dPCR) is a nucleic acid absolute quantification technology. Unlike qPCR, dPCR enables direct calculation of the number of DNA molecules, providing absolute quantification of the original sample. Unlike methods relying on the cycle threshold (Ct) of amplification curves, dPCR is independent of amplification efficiency, offering superior accuracy and reproducibility. It is widely applied in fields where Ct-based methods fall short, including copy number variation (CNV) analysis, mutation detection, gene expression studies, validation of next-generation sequencing (NGS) results, miRNA expression profiling, single-cell gene expression analysis.
PerfectStart® CHIPS dPCR SuperMix (AQ715)
This product is a 2×premixed solution designed for chip-based digital PCR, enabling amplification and absolute quantification of DNA samples. Simply add primers, probe and template to perform detection. It exhibits high amplification efficiency and anti-interference capability, demonstrating broad applicability in tumor and various pathogen detection. The dUTP/UDG in the SuperMix can function at room temperature to eliminate carry-over contamination caused by PCR products and aerosol to ensure the accuracy of results.
Application: Chip-based dPCR
Ultra-Low Host Nucleic Acid Residue
Commercial molecular enzymes are typically produced through expression in engineered bacterial strains (e.g., E. coli), which often results in residual host genomic DNA contamination. Additionally, external nucleic acids may be introduced during reagent preparation from environmental or human sources, leading to potential DNA contamination in enzyme products. During pathogen detection, these contaminating background nucleic acids may be co-amplified with target sequences, compromising the reliability of qPCR results.
PerfectStart® Taq Low DNA qPCR SuperMix UDG (AQ714)
Features
• Minimal host DNA residue: No detectable genomic DNA from 16 common pathogenic bacteria (including E. coli, P. aeruginosa, and S. aureus) in NTC controls.
• Excellent amplification efficiency: Achieves 100% efficiency across a broad dynamic range (10-10^5 copies/μl).
• Superior stability: Maintains stable performance under various conditions including repeated freeze-thaw cycles, pre-mixed reagent formulations, room temperature, and 37°C.
• dUTP/UDG system: Effectively prevents carry-over contamination caused by PCR products, ensuring accurate results.
Application
Specifically designed for applications requiring ultra-low host nucleic acid residue, including pathogen detection assays and quality control testing of biological products (particularly E. coli-derived preparations)
Contamination control
NTC amplification of E. coli and human gDNA systems using AQ714 and AQ712 demonstrated undetectable host DNA contamination in AQ714, confirming its ultra-low residual host DNA content.
Wide range of applications
AQ714 successfully amplifies ASFV, HPV-16, GAS, and Brucella, confirming wide template adaptability.
Lyophilizable Series
TransGen Biotech can provide lyophilizable formulations for full range of IVD raw materials, supporting multiple lyophilization forms including powder/microspheres in 8-tube strips or microspheres in vials. Traditional PCR reaction systems require low-temperature storage, making them susceptible to temperature fluctuations and contamination. This results in high transportation costs and operational inconveniences, limiting their application in resource-limited settings and point-of-care testing. In contrast, lyophilized PCR reagents offer enhanced stability, reduced cold-chain dependence, improved operational efficiency and accuracy and lower costs. These advantages have made lyophilized reagents increasingly popular.
• Comprehensive offerings: Full range of IVD raw materials available.
• Compatible with multiple lyophilization forms: Powder/microspheres in 8-tube strips or microspheres in vials.
• Excellent stability: Maintains performance after 45 days at 45°C, stable for 60 days at 37°C.
• Customization: Custom lyophilization solutions.
PerfectStart® II Probe qPCR Lyo-SuperMix UDG (AQ713)
Application
Lyophilizable version of AQ712. Suitable for probe-based qPCR detection of DNA from animals, plants, and microorganisms.
PerfectStart® III Fast Probe qPCR Lyo-SuperMix UDG (AQ723)
Application
Lyophilizable version of AQ722. Suitable for the detection of complex samples, validated applications include HPV, HBV, and animal pathogen detection.
PerfectStart® IV Fast Probe qPCR Lyo-SuperMix UDG (AQ733)
Application
Lyophilizable version of AQ732. Excellent performance for swabs, blood and inhibitor-containing samples, validated applications include African Swine Vever (samples with poor extraction), HPV, lung cancer, and respiratory bacteria detection.
Compatible with multiple lyophilization forms
Excellent stability
The lyophilized microspheres of AQ723, after being subjected to thermal acceleration at 37°C for 60 days and 0 days, were reconstituted and used for quadruplex amplification with medium and low concentrations of ASFV-gDNA as templates. The results showed no performance change after 60 days of thermal acceleration at 37°C, indicating excellent stability.
The three forms of AQ713 (lyophilized microspheres, lyophilized powder and pre-lyophilization solution) stored at 45°C for 45 days were used for gene amplification. The results demonstrated no performance change after 45 days of storage at 45°C, confirming stable product performance.
