Product Details
PerfectStartTM II Probe qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except probe, primer and
template. It contains PerfectStartTM Taq DNA Polymerase (PerfectStartTM Taq DNA polymerase is a hot-start Taq DNA polymerase
containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and
preventing non-specific amplification at low temperature), specially optimized dual-cation buffer, dNTPs, PCR enhancer and stabilizer.
qPCR SuperMix is provided at 2×concentration and can be used at 1×concentration by adding template, primer, probe, passive
reference dye (optional) and Nuclease-free Water. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA,
which can prevent cross contamination.
Highlights
• PerfectStart™ Taq DNA Polymerase, hot start with three antibodies blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
• Specially optimized qPCR reaction buffer provides higher sensitivity and specificity.
• dUTP/UDG used to avoid cross contamination.
• Passive reference dyes are provided for different qPCR instruments.
Storage
at -18℃ or below for two years
Shipping
Dry ice (-70°C)

Comparison to other brands

Using products from TransGen, Company V, and Company T, amplify different concentrations of porcine reproductive and respiratory syndrome virus (PRRSV) cDNA (10 pg, 0.1 pg, 0.01 pg) and bovine viral diarrhea virus (BVDV) cDNA (100 pg, 1 pg, 0.1 pg) as templates, conduct qPCR detection, and analyze the amplification effect and sensitivity.
