TransStart^® FastPfu DNA Polymerase -AP221

  Product Name  

TransStart^® FastPfu DNA Polymerase(AP221)

  Patent  

Patent Number：ZL201510271941.3    ZL2023113204561    ZL2023118400050

  High-Impact Factor Literature Collection  

•Lei Z, Meng H, Liu L, et al. Mitochondrial base editor induces substantial nuclear off-target mutations[J]. Nature, 2022.(IF 69.50)

•Zhang H, Zhu Y, Liu Z, et al. A volatile from the skin microbiota of flavivirus-infected hosts promotes mosquito attractiveness[J]. Cell, 2022.(IF 66.85)

•Wang Y, Zhang S, Yang X, et al. Mesoscale DNA feature in antibody-coding sequence facilitates somatic hypermutation[J]. Cell, 2023.(IF 64.50)

•Lin Q, Jin S, Zong Y, et al. High-efficiency prime editing with optimized, paired pegRNAs in plants[J]. Nature Biotechnology, 2021.(IF 54.90)

•Zong Y, Liu Y, Xue C, et al. An engineered prime editor with enhanced editing efficiency in plants[J]. Nature Biotechnology, 2022.(IF 54.00)

•Liu J L, Yan X Q, Wu H, et al. RNA codon expansion via programmable pseudouridine editing and decoding[J]. Nature, 2025.(IF 48.50)

•Yang J, Zhao T, Fan J, et al. Structure-guided discovery of bile acid derivatives for treating liver diseases without causing itch[J]. Cell, 2024.(IF 45.60)

•Jin S, Lin Q, Luo Y, et al. Genome-wide specificity of prime editors in plants[J]. Nature Biotechnology, 2021.(IF 36.55)

•Wang S, Zong Y, Lin Q, et al. Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9[J]. Nature biotechnology, 2020.(IF 36.55)

•Li C, Zhang R, Meng X, et al. Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors[J]. Nature biotechnology, 2020.(IF 35.72)

•Lin Q, Zong Y, Xue C, et al. Prime genome editing in rice and wheat[J]. Nature biotechnology, 2020.(IF 31.90)

•Song B, Chen Y, Liu X, et al. Ordered assembly of the cytosolic RNA-sensing MDA5-MAVS signaling complex via binding to unanchored K63-linked poly-ubiquitin chains[J]. Immunity, 2021.(IF 31.75)

•Li Y, Zhao L, Zhang Y, et al. Structural basis for product specificities of MLL family methyltransferases[J]. Molecular Cell, 2022.(IF 19.33)

•Li C, Zong Y, Jin S, et al. SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds[J]. Genome biology, 2020.(IF 18.36)

•Zhang H, Li Z, Zhou S, et al. A fungal NRPS-PKS enzyme catalyses the formation of the flavonoid naringenin[J]. Nature Communications, 2022.(IF 17.69)

•Lei Y, Fei P, Song B, et al. A loosened gating mechanism of RIG-I leads to autoimmune disorders[J]. Nucleic acids research, 2022.(IF 16.97)

•Fan J, Ran H, Wei P L, et al. An ortho-Quinone Methide Mediates Disulfide Migration in the Biosynthesis of Epidithiodiketopiperazines[J]. Angewandte Chemie International Edition, 2023.(IF 16.82)

•Fan J, Ran H, Wei P L, et al. Pretrichodermamide A Biosynthesis Reveals the Hidden Diversity of Epidithiodiketopiperazines[J]. Angewandte Chemie, 2023.(IF 16.82)

•Song Z, Zhou S, Zhang H, et al. Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex[J]. Nature Communications, 2023.(IF 16.60)

•Jin S, Fei H, Zhu Z, et al. Rationally designed APOBEC3B cytosine base editors with improved specificity[J]. Molecular cell, 2020.(IF 15.58)

•Lin R, Liang J, Wang R, et al. The raphe dopamine system controls the expression of incentive memory[J]. Neuron, 2020.(IF 14.40)

  Features  

• Fast: Features a fast extension rate of 4 kb per minute.

• High Fidelity: Demonstrates 54-fold higher fidelity than standard Taq polymerases and 3-fold higher fidelity than standard Pfu polymerases.

• Long-Range Amplification: Capable of amplifying genomic DNA fragments up to 15 kb and plasmid DNA fragments up to 20 kb.

• High Specificity: Utilizes the novel "TransStart" dual-block hot-start technology, which blocks both primers and templates to minimize non-specific amplification.

• Robust Performance: Incorporates a unique PCR stimulant that enhances the enzyme's ability to amplify complex templates.

• Blunt-Ended Products: The amplification generates blunt-ended PCR products, enabling direct cloning into pEASY^® -Blunt series vectors.

  Data Presentation  

High Processivity

PCR Using different types of template and amplify fragments of various length

  Product Information