pEASY Cloning Vectors
  • CU101-01.JPG
CU101-01.JPG

pEASY® -Uni Seamless Cloning and Assembly Kit


Product Details

This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector.

•Fast: 15 minutes.
•Broad: no restriction enzyme digestions. Can be cloned into any sites.
•High efficiency: up to 95% cloning efficiency.
•Seamless: no extra sequences introduced; up to 5 fragments assembly.

Storage

Trans1-T1 Phage Resistant Chemically Competent Cells at -70°C for six months; others at -20°C for one year.


Shipping
Dry ice (-70℃)

Product Contents


ComponentCU101-01CU101-02CU101-03
2×Assembly Mix50 μl100 μl3×100 μl
Linearized pUC19 Control Vector (10 ng/μl)3 μl6 μl18 μl
Control Insert (1 kb,20 ng/μl)3 μl6 μl18 μl
Trans1-T1 Phage Resistant Chemically Competent Cell5×100 μl10×100 μl30×100 μl


Citations

Wang Y , Tao F , Xin B , et al. Switch of metabolic status: redirecting metabolic flux for acetoin production from glycerol by activating a silent glycerol catabolism pathway[J]. Metabolic Engineering, 2017, 39:90-101. 

Lin B , Zhuo K , Chen S , et al. A novel nematode effector suppresses plant immunity by activating host reactive oxygen species‐scavenging system[J]. New Phytologist, 2016, 209(3):1159-73.

Wang R , Peng J , Li Q X , et al. Phosphorylation-Mediated Regulatory Networks in Mycelia of, Pyricularia oryzae, Revealed by Phosphoproteomic Analyses[J]. Molecular & Cellular Proteomics, 2017 , 16(9):1669-1682.

Li S , Wang H , Li F , et al. The maize transcription factor, EREB58, mediates the jasmonate-induced production of sesquiterpene volatiles[J]. The Plant Journal, 2015, 84(2):296-308.

Hu H , Wang W , Tang H , et al. Characterization of Pseudooxynicotine Amine Oxidase of Pseudomonas putida S16 that Is Crucial for Nicotine Degradation[J]. Scientific Reports, 2015, 5:17770.


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