pEASY Cloning Vectors
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pEASY® -Uni Seamless Cloning and Assembly Kit

Product Details

This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector.

•Fast: 15 minutes.
•Broad: no restriction enzyme digestions. Can be cloned into any sites.
•High efficiency: up to 95% cloning efficiency.
•Seamless: no extra sequences introduced; up to 5 fragments assembly.


Trans1-T1 Phage Resistant Chemically Competent Cells at -70°C for six months; others at -20°C for one year.

Dry ice (-70℃)

Product Contents

2×Assembly Mix50 μl100 μl3×100 μl
Linearized pUC19 Control Vector (10 ng/μl)3 μl6 μl18 μl
Control Insert (1 kb,20 ng/μl)3 μl6 μl18 μl
Trans1-T1 Phage Resistant Chemically Competent Cell5×100 μl10×100 μl30×100 μl


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