Supporting Scientific Research, Transgen RNA Purification and Reverse Transcription SuperMix were cited in Nature

Time:2023-12-29 21:10:41 View:

Article information

Article title: Molecular basis of methyl-salicylate-mediated plant airborne defense

Journal: Nature

Date of Publication: September 13, 2023

Article link:

Using TransGen products:

TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311)

TransZol (ET101)


Research background

Plants produce volatile organic compounds (VOCs) in response to environmental stimuli, which can induce defense reactions in neighboring plants. This phenomenon is known as airborne defense (AD) or plant-plant communication (PPC). Although this communication has been observed in various plant species for decades, the molecular mechanisms underlying VOC-mediated PPC have remained unclear. Additionally, apart from ethylene receptors, the receptors through which plants perceive other VOCs have yet to be identified.

Aphids are one of the most destructive agricultural pests worldwide, and their biting triggers the release of VOCs, including methyl salicylate (MeSA), from plants. MeSA plays a crucial role in plant defense against herbivorous insects such as aphids, but how MeSA acts as a signaling molecule for interplant communication and activates AD defense against aphids remains a long-standing unresolved question. It is also unclear whether plants possess receptors to recognize and perceive airborne MeSA. Furthermore, the potential interference of aphids and viruses with plant airborne defense is unknown.


Aphids transmit viruses and are destructive crop pests1. Plants that have been attacked by aphids release volatile compounds to elicit airborne defence (AD) in neighbouring plants. However, the mechanism underlying AD is unclear. Here we reveal that methyl-salicylate (MeSA), salicylic acid-binding protein-2 (SABP2), the transcription factor NAC2 and salicylic acid-carboxylmethyltransferase-1 (SAMT1) form a signalling circuit to mediate AD against aphids and viruses. Airborne MeSA is perceived and converted into salicylic acid by SABP2 in neighbouring plants. Salicylic acid then causes a signal transduction cascade to activate the NAC2–SAMT1 module for MeSA biosynthesis to induce plant anti-aphid immunity and reduce virus transmission. To counteract this, some aphid-transmitted viruses encode helicase-containing proteins to suppress AD by interacting with NAC2 to subcellularly relocalize and destabilize NAC2. As a consequence, plants become less repellent to aphids, and more suitable for aphid survival, infestation and viral transmission. Our findings uncover the mechanistic basis of AD and an aphid–virus co-evolutionary mutualism, demonstrating AD as a potential bioinspired strategy to control aphids and viruses.


Plant Systemic Acquired Resistance (SAR) Molecular Mechanism Schematic Diagram.

TransGen Product Support

High-quality reagents are essential tools for scientific research. Transgen's RNA purification product TransZol (ET101) and reverse transcription product TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311) greatly support this study.


TransZol (ET101)

This product utilizes guanidine isothiocyanate to lyse cells, making it suitable for the rapid extraction of total RNA from various tissues and cells. It offers greater safety during operation, has a wide range of applications, and allows for the easy obtainment of high-quality RNA within one hour.


• High operational safety: RNA Extraction Agent is used instead of chloroform.

• Wide range of applications: suitable for small samples (50-100 mg tissue, 5×106 cells), or large samples (≥1 g tissue or ≥107 cells) for human, animal, plant, blood and bacterial tissue extraction both apply.

• Fast extraction: the reaction can be completed within an hour.

• Visualization of operation: The solution is pink for easy separation of aqueous and organic phases.

• High extraction purity: minimal DNA and protein contamination.

• RNA lysate: facilitates RNA preservation and reduces inhibition of reverse transcription reactions.


TransGen products have once again been published in the Nature journal, which proves customer's recognition of the quality and strength of the TransGen products, and also perfectly interprets the principle of " Quality is the highest priority. To serve the customers with superior quality and service. " that the TransGen has always adhered to. TransGen has always been on the road of helping scientific research, and hopes to work side by side with more scientific researchers in the future and continue to help scientific research with more and better products.


TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311)


• In the same reaction system, simultaneous reverse transcription and removal of genomic DNA are performed, making the operation simple and reducing the risk of contamination.

• Product used for qPCR: 15 minutes for reverse transcription; Product used for PCR: 30 minutes for reverse transcription.

• After the reaction is completed, simultaneous heat inactivation of RT/RI and gDNA Remover is performed. Compared to the traditional method of pretreating RNA with DNase I, it avoids the damage to RNA caused by heat inactivation of DNase I after treatment.

• Simple operation.

• cDNA up to 12 kb.


Some articles published using TransZol (ET101):

•Gong Q, Wang Y J, He L F, et al. Molecular basis of methyl salicylate-mediated plant airborne defense [J]. Nature, 2023.

•He C, Xing F, Liang J, et al. The ABI5-dependent down-regulation of mitochondrial ATP synthase OSCP subunit facilitates apple necrotic mosaic virus infection[J]. Journal of Experimental Botany, 2023.

•Hong Y, Xia H, Li X, et al. Brassica napus BnaNTT1 modulates ATP homeostasis in plastids to sustain metabolism and growth[J]. Cell Reports, 2022.

•Wang S, Lu M, Wang W, et al. Macrophage Polarization Modulated by NF‐κB in Polylactide Membranes‐Treated Peritendinous Adhesion[J]. Small, 2022.

•Wang Y, Gong Q, Wu Y, et al. A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants[J]. Cell Host & Microbe, 2021.

•Zhao M, Wang B, Zhang C, et al. The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson’s disease[J]. Cell Death & Differentiation, 2021.

•Hao M, Hou S, Li W, et al. Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy[J]. Science Translational Medicine, 2020.

•Gao L, Guo Q, Li X, et al. MiR-873/PD-L1 axis regulates the stemness of breast cancer cells[J]. EBioMedicine, 2019.

•Chen K, Hu Z, Song W, et al. Diversity of O-glycosyltransferases contributes to the biosynthesis of flavonoid and triterpenoid glycosides in Glycyrrhiza uralensis[J]. ACS Synthetic Biology, 2019.

•Hu Z, He J, Chen K, et al. Molecular cloning and biochemical characterization of a new flavonoid glycosyltransferase from the aquatic plant lotus[J]. Biochemical and biophysical research communications, 2019.

•Wang Z, Wang S, Xu Z, et al. Highly promiscuous flavonoid 3-O-glycosyltransferase from Scutellaria baicalensis[J]. Organic letters, 2019.


Some articles published using TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311):

•Gong Q, Wang Y J, He L F, et al. Molecular basis of methyl salicylate-mediated plant airborne defense [J]. Nature, 2023.

•Guan J, Wang G, Wang J, et al. Chemical reprogramming of human somatic cells to pluripotent stem cells[J]. Nature, 2022.

•Chen J, Ou Y, Luo R, et al. SAR1B senses leucine levels to regulate mTORC1 signalling[J]. Nature,2021.

•Chen J, Ou Y, Yang Y, et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018.

•Fan H, Quan S, Ye Q, et al. A molecular framework underlying low-nitrogen-induced early leaf senescence in Arabidopsis thaliana[J]. Molecular Plant, 2023.

•Yan Y, Sun J, Ji K, et al. High incidence of the virus among respiratory pathogens in children with lower respiratory tract infection in northwestern China[J]. Journal of Medical Virology, 2023.

•Liu W, Yao Q, Su X, et al. Molecular insights into Spindlin1-HBx interplay and its impact on HBV transcription from cccDNA minichromosome[J]. Nature Communications, 2023.

•Guo Z, Cao H, Zhao J, et al. A natural uORF variant confers phosphorus acquisition diversity in soybean[J]. Nature Communications, 2022.

•Wang B, Zhao M, Su Z, et al. RIIβ‐PKA in GABAergic Neurons of Dorsal Median Hypothalamus Governs White Adipose Browning[J]. Advanced Science, 2022.

• Liu S, Liu C, Lv X, et al. The chemokine CCL1 triggers an AMFR-SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis[J]. Immunity, 2021.

•Wang R, Xue Y, Fan J, et al. A systems genetics approach reveals PbrNSC as a regulator of lignin and cellulose biosynthesis in stone cells of pear fruit[J]. Genome Biology, 2021.

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