Uracil-DNA Glycosylase (Low Temperature)
Catalog Number： LU201-01
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Product Details
Low temperature Uracil-DNA Glycosylase (UDG) is a recombinant protein derived from psychrophile marine bacteria that have been modified to be expressed in Escherichia coli. UDG enzyme can effectively hydrolyze the N-glycosidic bond between the uracil (dU) base and deoxyribose in single or double-stranded DNA, thus releasing free uracil and producing pyrimidine deficiency sites, which are mainly used to eliminate the aerosol contamination caused by PCR products containing dU. This product is easily hydrolyzed and fractured at high temperatures or pH. Compared with conventional UDG enzymes, the product can operate at a lower temperature (20°C), i.e., when preparing qPCR or qRT-PCR reaction systems at room temperature, it can degrade contaminated dU-containing templates. This enzyme does not degrade dU-containing cDNA (DNA/RNA hybrid form) produced during reverse transcription. This product has high activity in most PCR reaction buffers and is suitable for PCR/qPCR, RT-PCR/qRT-PCR, and LAMP/RT-LAMP.
Applications
Remove uracil base from single-stranded or double-stranded DNA; prevent aerosol contamination of PCR products containing dU and improve the specificity of the reaction.
Strong Digestion Capability
Using the U-PCR products amplified by African Swine Fever Virus (ASFV) and Pseudorabies Virus (PRV), as well as the U-PCR products amplified from the novel coronavirus and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) after reverse transcription, as templates, add 0 UDG, TransGen UDG (2.5 U/mL), and Company N UDG (2.5 U/mL) for digestion, then perform qPCR to detect the digestion effect. The results show that TransGen UDG has strong digestion ability, effectively removing non-template DNA contamination in the system. (ΔCq represents the difference in Cq values between TransGen UDG, Company N UDG, and 0 UDG reaction systems. A higher difference indicates a stronger UDG digestion capability.)
