This product utilizes the TaqMan probe-based qPCR method to the detection of mycoplasma DNA for qualitative identification of mycoplasma contamination. It offers a broad detection range covering various species within the class Mollicutes, including Mycoplasma spp., Acholeplasma spp., and Spiroplasma spp. The TransDetect® qPCR Mycoplasma SuperMix II included in the kit contains hot-start Taq DNA polymerase, a qPCR reaction buffer specially optimized for mycoplasma detection, dNTPs, PCR enhancer, and stabilizer. In addition, a dUTP/UDG system is introduced into this reaction mix, which can degrade U-containing ssDNA and dsDNA prior to amplification, eliminating carry-over contamination caused by PCR products. The Myco Primer & Probe Mix II includes primers and probes for amplification of both mycoplasma sequences and the Myco Internal Control Template II. The FAM channel detects mycoplasma-specific amplification, while the ROX channel monitors the amplification of the Myco Internal Control Template II. This kit can be used directly to detect mycoplasma in samples such as cell culture supernatants, cell culture media, and serum.

Storage

at -18℃ or below for two years in dark

Shipping

Dry ice (-70℃)