p24 is a highly conserved and most abundant major structural protein in HIV virions. It is a product encoded by the  structural gene gag and plays a key role in the packaging and maturation of the virus. Lentiviral vector is a viral vector  system modified on the basis of HIV-1 virus, which can efficiently transfer the target gene into cells. The content of  p24 protein represents the number of lentivirus particles, therefore, the physical titer of lentivirus can be determined  by assaying the p24 content by ELISA method.  This kit uses one-step Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) to rapidly measure the amount of  HIV-p24 in lentiviral supernatant. The entire incubation time is 70 minutes. The ELISA microplate in the kit is  precoated with the HIV-p24 antibody. The virus lysate, standard/test sample, biotin-labeled HIV-p24 detection  antibody and horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) are added to the microplate wells. After  incubation, "coated antibody-HIV-p24 protein-detection antibody-Streptavidin-HRP" immune complex can be  produced. After washing, the chromogenic substrate TMB is added to the wells. HRP catalyzes the TMB substrate to  create blue coloration, with intensity positively correlated with the concentration of HIV-p24 in the sample. The  reaction is stopped by adding stop solution, and the absorbance is measured at 450 nm (reference wavelength 570-630  nm). A standard curve is drawn to calculate the concentration of HIV-p24 in the sample based on the absorbance  value. This kit is highly specific, has high detection sensitivity and is convenient to use.

Storage: 

at 2~8°C in the dark for one year.

Shipping：

ice bag (4℃)