p24 is a highly conserved and most abundant major structural protein in HIV virions. It is a product encoded by the structural gene gag and plays a key role in the packaging and maturation of the virus. Lentiviral vector is a viral vector system modified on the basis of HIV-1 virus, which can efficiently transfer the target gene into cells. The content of p24 protein represents the number of lentivirus particles, therefore, the physical titer of lentivirus can be determined by assaying the p24 content by ELISA method.

This kit uses Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) to measure the amount of HIV-p24 in lentiviral supernatant. The ELISA microplate in the kit is precoated with a high-affinity anti-human HIV-p24 antibody. The virus lysate and standard or test sample are added to the microplate wells. After incubation, HIV-p24 present in the sample will be bound specifically to the precoated antibody on the microplate wells. After washing, biotin-labeled anti-human HIV-p24 detection antibody is added to the microplate wells. After re-incubation, the detection antibody will be bound specifically to HIV-p24 anchored on the microplate wells. Subsequently, the horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is added to the microplate wells and incubated. Biotin and streptavidin on the detection antibody produces the "coated antibody-HIV-p24 protein-detection antibody-Streptavidin-HRP" immune complex through high-strength non-covalent binding. After washing again, the chromogenic substrate TMB is added to the wells. HRP catalyzes the TMB substrate to create blue coloration, with intensity positively correlated with the concentration of HIV-p24 in the sample. The reaction is stopped by adding stop solution, and the absorbance is measured at 450 nm (reference wavelength 570-630 nm). A standard curve is drawn to calculate the concentration of HIV-p24 in the sample based on the absorbance value. This kit is highly specific, has high detection sensitivity and is convenient to use.

Storage

at 2~8°C in the dark for one year

Shipping

ice bag (4℃)