2×TransTaq® High Fidelity (HiFi) PCR SuperMix II (-dye)
Catalog Number： AS131-21
Price：Please inquire first
Product Details
This product is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase with hot start, dNTPs and optimized buffer. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O. Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T series vectors.
• Reduced reaction setup time.
• Minimized contamination caused by multi-step operation.
• TransTaq® High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Applications
• GC/AT-rich templates, complex templates and long PCR.
• TransTaq® HiFi PCR SuperMix I is optimized for the amplification of genomic DNA (≤ 15 kb).
• TransTaq® HiFi PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Storage
-18°C or below for two years
Shipping
Dry ice (-70 ℃)
| Component | AS131-01/21 | AS131-02/22 |
| 2×TransTaq® HiFi PCR SuperMix (Mix I)/(Mix II) | 1 ml | 5×1 ml |
| Nuclease-free Water | 1 ml | 5 ml |
1 Guo C J, Ma X K, Xing Y H, et al. Distinct processing of lncRNAs contributes to non-conserved functions in stem cells[J]. Cell, 2020.(IF 36.21)
