TransNGS® Whole Transcriptome Amplification Kit
Catalog Number： KC921-01
Price：Please inquire first
Product Details
TransNGS® Whole Transcriptome Amplification Kit is capable of obtaining amplified full-length cDNA from single cell. It is suitable for amplification and library construction of full transcriptome from 1-105 cells or 10 pg-100 ng total RNA. The kit uses WTA Oligo(dT) as the reverse transcription primer and employs a reverse transcriptase with high synthesis efficiency and template-switching activity to add a special sequence to the 3' end of the cDNA, thereby obtaining full-length cDNA products. The kit is compatible with various cell types and tissue types, suitable for cell materials with varying RNA content. Typically, a single-cell library can yield 10-60 ng of full transcriptome amplification products.
• Strong compatibility with different cell types, suitable for cells with low RNA content (such as immune cells).
• Strong compatibility with different tissue types, suitable for tissues that are difficult to dissociate (such as brain tissue).
• High yield in constructing single-cell libraries, excellent peak shapes, high efficiency in gene detection (FPKM > 1).
• The compatible sample volume is up to 6 μl, which can be adapted to different concentrations of RNA or different numbers of cells
WTA TSO should be stored at -70℃ or below for one year. The other components should be stored at -18℃ or below for one year.
Dry ice (-70℃)
Component | KC921-01 (12 rxns) | KC921-02 (96 rxns) |
| WTA Lysis Buffer II | 12 μl | 96 μl |
| WTA Oligo(dT) | 24 μl | 192 μl |
| 10 mM dNTPs | 24 μl | 192 μl |
| Ribonuclease Inhibitors | 12 μl | 96 μl |
| WTA RT Buffer | 48 μl | 384 μl |
| DTT | 24 μl | 192 μl |
| WTA TSO | 12 μl | 96 μl |
| WTA Reverse Transcriptase II | 24 μl | 192 μl |
| 2×WTA Amplification SuperMix II | 600 μl | 4.8 ml |
| WTA PCR Primer | 24 μl | 192 μl |
| RNase-free Water | 1 ml | 2×4 ml |
