TransScript® Uni One-Step gDNA Removal and cDNA Synthesis SuperMix
Catalog Number： AU311-02
Price：Please inquire first
Product Details
TransScript®-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature(42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds.
• Broad range reaction temperature (42°C -65°C ) .
• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 20 kb.
Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
• cDNA library construction, primer extension, 3’ and 5’ RACE
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AU311-02 (50 rxns) | AU311-03 (100 rxns) |
| TransScript® -Uni RT/RI Enzyme Mix | 50 μl | 100 μl |
| gDNA Remover | 50 μl | 100 μl |
| 2×TS-Uni Reaction Mix | 500 μl | 1 ml |
| Random Primer(N9) (0.1 μg/μl) | 50 μl | 100 μl |
| Anchored Oligo(dT)20 Primer (0.5 μg/μl) | 50 μl | 100 μl |
| RNase-free Water | 500 μl | 1 ml |
1 Li C, Guan H, Jing X, et al. Genomic insights into historical improvement of heterotic groups during modern hybrid maize breeding[J]. Nature Plants, 2022.(IF 17.35)
2 Chen L, Zhang C, Ma W, et al. METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stability[J]. Nucleic Acids Research, 2022.(IF 19.16)
3 Wang Y, Gong Q, Wu Y, et al. A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants[J]. Cell Host & Microbe, 2021.(IF 21.02)
4 Kumar S, Wang G, Liu W, et al. Hypoxia-induced mitogenic factor promotes cardiac hypertrophy via calcium-dependent and hypoxia-inducible factor-1α mechanisms[J]. Hypertension, 2018.(IF 6.82)
