One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. TransScript® RT and TransTaq® HiFi DNA Polymerase are used in the kit.
Amplification of fragment up to 8 kb.
Applications
Multiple copy gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Dong Z , Yu Y , Li S , et al. Abscisic Acid Antagonizes Ethylene Production through the ABI4-Mediated Transcriptional Repression of ACS4 and ACS8 in Arabidopsis[J]. Molecular Plant (Cell Press), 2016, 9(1):126-135.
Shoufang J , Jing S , Sanqiao Y , et al. Fluoride and Arsenic Exposure Impairs Learning and Memory and Decreases mGluR5 Expression in the Hippocampus and Cortex in Rats[J]. PLoS ONE, 2014, 9(4):e96041.
The protective effect of piperine on dextran sulfate sodium induced inflammatory bowel disease and its relation with pregnane X receptor activation[J]. Journal of Ethnopharmacology, 2015, 169:109-123.
Su Y , Yan R , Duan Z , et al. Prevalence and risk factors of hepatitis C and B virus infections in hemodialysis patients and their spouses: A multicenter study in Beijing, China[J]. Journal of Medical Virology, 2013, 85(3):425-432.
Zhou H , Yang J , Xin T , et al. Exendin-4 enhances the migration of adipose-derived stem cells to neonatal rat ventricular cardiomyocyte-derived conditioned medium via the phosphoinositide???3-kinase/Akt-stromal cell-derived factor-1α/CXC chemokine receptor 4 pathway[J]. Molecular Medicine Reports, 2015,11(6):4063-72.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
