Product Details
TransScript® IV Reverse Transcriptase is a modified M-MLV reverse transcriptase. The product uses RNA as a template to synthesize the first-strand cDNA, meanwhile, remove the remaining genomic DNA in the RNA template in the same reaction volume. After the reaction, it only needs to heat at 85°C for 5 seconds to inactivate TransScript® IV RT/RI Enzyme Mix and gDNA Remover at the same time. The optimal reaction temperature of the enzyme is 50 °C, and it has ultra thermal stability
Applications
·High-copy number and low-copy number gene detection.
·High GC or complex secondary structure RNA template.
·For difficult-to-handle RNA sample: degraded RNA template and RNA template containing RT enzyme inhibitors.
·cDNA library construction, primer extension, 3’ and 5’ RACE.
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AW311-02 (50 rxns) | AW311-03 (100 rxns) |
| TransScript® IV RT/RI Enzyme Mix | 50 μl | 100 μl |
| gDNA Remover | 50 μl | 100 μl |
| 2×TS IV Reaction Mix | 500 μl | 1 ml |
| Random Primer (N9) (0.1 μg/μl) | 50 μl | 100 μl |
| Anchored Oligo(dT)20 Primer (0.5 μg/μl) | 50 μl | 100 μl |
| RNase-free Water | 500 μl | 1 ml |
