One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. TransScript® II RT and TransTaq® HiFi DNA Polymerase are used in the kit.
Amplification of fragment up to 8 kb.
Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Xiao T , Wang Y , Luo H , et al. A differential sequencing-based analysis of the C. elegans noncoding transcriptome[J]. RNA, 2012, 18(4):626-639.
Fan Z , Chen X , Chen R . Transcriptome-wide analysis of TDP-43 binding small RNAs identifies miR-NID1 (miR-8485), a novel miRNA that represses NRXN1 expression[J]. Genomics, 2014, 103(1):76-82.
Zhao F , Ma J , Li L , et al. GhNAC12, a neutral candidate gene, leads to early aging in cotton (Gossypium hirsutum L)[J]. Gene, 2015, 576(1P2):268-274.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
