Product Details
TransScript® II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript® II RT/RI Enzyme Mix and 2×TS II Reaction Mix at 42-55℃, of which the optimum temperature is 50℃.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis to ensure its synthesis length and yield.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)20 Primer is specifically designed to bind to the first base next to the 5' end of Poly(A) tail of mRNA, providing higher specificity and high efficiency for first-strand cDNA synthesis.
• cDNA up to 15 kb.
Applications
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AH301-02 | AH301-03 |
| TransScript® II RT/RI Enzyme Mix | 50 μl | 100 μl |
| 2×TS II Reaction Mix | 500 μl | 1 ml |
| Random Primer (N9) (0.1 μg/μl) | 50 μl | 100 μl |
| Anchored Oligo(dT)20 Primer (0.5 μg/μl) | 50 μl | 100 μl |
| RNase-free Water | 500 μl | 1 ml |
1 Zhao X, Lian X, Xie J, et al. Accumulated cholesterol protects tumours from elevated lipid peroxidation in the microenvironment[J]. Redox Biology, 2023.(IF 10.78)
