One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. EasyScript® RT and TransTaq®HiFi DNA Polymerase are used in the kit.
Amplification of fragment up to 4 kb.
Applications
Multiple copy gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Dongfang H , Lin L , Jinyuan G, et al. Genetic Diversity and Positive Selection Analysis of Classical Swine Fever Virus Envelope Protein Gene E2 in East China under C-Strain Vaccination[J]. Frontiers in Microbiology, 2016, 5(7):85.
Ma X , Gu L , Li H , et al. Hypoxia-induced overexpression of stanniocalcin-1 is associated with the metastasis of early stage clear cell renal cell carcinoma[J]. Journal of Translational Medicine, 2015, 13(1):56.
Rotimi OA, Rotimi SO, Duru CU, et al. Acute aflatoxin B1 – Induced hepatotoxicity alters gene expression and disrupts lipid and lipoprotein metabolism in rats[J]. Toxicol Report. 2017,24(4): 408-414.
Mao L , Li W , Liu X , et al. Chinese border disease virus strain JSLS12-01 infects piglets and down-regulates the antibody responses of classical swine fever virus C strain vaccination[J]. Vaccine, 2015, 33(32):3918-3922.
Liu Z Z , Xie X D , Qu S X , et al. Small breast epithelial mucin (SBEM) has the potential to be a marker for predicting hematogenous micrometastasis and response to neoadjuvant chemotherapy in breast cancer[J]. Clinical & Experimental Metastasis, 2010, 27(4):251-259.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
