Product Details
One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. EasyScript® RT and TransTaq®HiFi DNA Polymerase are used in the kit.
Features
Amplification of fragment up to 4 kb.
Applications
Multiple copy gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Product Composition
| Component | AE411-02 (200 rxns) |
| EasyScript® One-Step Enzyme Mix | 80 μl |
| 2×ES One-Step Reaction Mix | 2×1 ml |
| RNase-free Water | 2×1 ml |
References
1 Zhu X, Yang M, Zhao P, et al. Catenin α 1 mutations cause familial exudative vitreoretinopathy by overactivating Norrin/β-catenin signaling[J]. The Journal of clinical investigation, 2021.(IF 11.86)
FAQ
