Products

Restriction Enzymes
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内切酶系列.JPG

FlyCut® SacI


Product Details

FlyCut® SacI is expressed and purified from E.coli that carries the recombinant SacI gene. The molecular weight is 40.8 kDa, with the recognition site at GAGCT^C. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes.This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...GAGCT↓C...3’
3’...C↑TCGAG...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentJS101-01JS101-02
FlyCut® SacI1,000 units2×1,000 units
10×FlyCut® Buffer250 μl500 μl
10×DNA Loading Buffer1 ml1 ml


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