FlyCut® NotI
Catalog Number: JN401-01
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Product Details
FlyCut® NotI is expressed and purified from E.coli that carries the recombinant Not! gene. The molecular weight is 43.3 kDa, with the recognition site at GC^GGCCGC. The reaction is conducted for 5-15 minutes at 37°C, and heat-inactivated at 65°C for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Enzyme Properties
Fast digestion in 5-15 minutes with high fidelity
Application
Genomic DNA, plasmid DNA, PCR product