Product Details
FlyCut® NheI is expressed and purified from E.coli that carries the recombinant NheI gene. The molecular weight is 39.1 kDa, with the recognition site at G^CTAGC. The reaction is conducted at 37oC, and heat-inactivated at 80oC for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Enzyme Properties
· Fast digestion in 5 minutes
· Universal buffer
· Star activity-free
Application
Genomic DNA, plasmid DNA, PCR product
Storage
at -20oC for two years
Shipping
Dry ice (-70℃)
Product Composition
