Product Details
FlyCut® EagI is expressed and purified from E.coli that carries the recombinant EagI gene. The molecular weight is 35.1 kDa, with the recognition site at C^GGCCG. The reaction is conducted for 5-15 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Enzyme Properties
· Fast digestion in 5 minutes
· Universal buffer
· Star activity-free
Application
Genomic DNA, plasmid DNA, PCR product
Product Composition
