This product is used for quantitative detection of Pichia DNA remaining in various biological products. The principle of this product is that the amount of nucleic acid in the sample is determined by the detection of the intensity of fluorescence which is proportional to the amount of amplified product during the amplification process by adding a fluorescent probe (TaqMan or Molecular Beacon, etc.) to the PCR system. Pichia qPCR SuperMix (2×) in this product contains PerfectStart Taq hot start enzyme (uses 3 monoclonal antibodies to efficiently bind with Taq DNA Polymerase, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature), qPCR reaction buffer, dNTPs, PCR enhancer and stabilizer specially optimized for Pichia DNA detection. In addition, a dUTP/UDG system is introduced into this reaction mix, which can degrade U-containing ssDNA and dsDNA before reverse transcription, eliminating cross-contamination caused by PCR products.
Features
• High specificity, high sensitivity, high amplification efficiency enabled by 3 kinds of blocking antibodies, and suitable for a wide range of samples.
• Specially optimized qPCR reaction buffer to provide higher extension speed, sensitivity and specificity.
• The use of UDG enzyme and dUTP can effectively prevent cross-contamination of PCR products to ensure accurate data.
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Name | DH141-01 | Component |
| Pichia qPCR SuperMix (2×) | 2 × 750 μl | PCR enzymes, dNTPs, Mg2+, PCR buffer, etc.
|
| 6 × Pichia Primer Probe Mix | 500 μl | Pichia DNA primer probe
|
| Pichia DNA Standard S0 | 100 μl | Pichia standard DNA |
| Standard Diluent | 3 ×1 ml |
|
| Nuclease-free Water | 1 ml
|
|
PCR enzymes, dNTPs, Mg2+, PCR buffer, etc.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
