This product is used for the quantitative detection of SV40LTA and E1A DNA in cell lines such as 293T in various biological products.
The principle of this product is that the amount of nucleic acid in the sample is determined by the detection of the intensity of fluorescence which is proportional to the amount of amplified product during the amplification process by adding a fluorescent probe (TaqMan or Molecular Beacon, etc.) to the PCR system. SV40LTA & E1A qPCR SuperMix (2×) in this product contains PerfectStart Taq hot start enzyme (uses 3 monoclonal antibodies to efficiently bind with Taq DNA Polymerase, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature), qPCR reaction buffer, dNTPs, PCR enhancer and stabilizer specially optimized for SV40LTA & E1A DNA detection. In addition, a dUTP/UDG system is introduced into this reaction mix, which can degrade U-containing ssDNA and dsDNA before reverse transcription, eliminating cross-contamination caused by PCR products.
Features
· High specificity, high sensitivity, high amplification efficiency enabled by 3 kinds of antibodies blocking, and suitable for a wide range of samples.
· Specially optimized qPCR reaction buffer to provide higher extension speed, sensitivity and specificity.
· The use of UDG enzyme and dUTP can effectively prevent cross-contamination of PCR products to ensure accurate data.
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
