ProteinFind® Anti-Histone H3 Mouse Monoclonal Antibody
Catalog Number： HL102-01
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Product Details
Histone H3 is an important component of the nucleosome, which, along with H2A, H2B, and H4, forms a heteroctamer around which the DNA tightly wraps to form a nucleosome[1]. Histone H3 plays an important role in cell proliferation, transcription regulation, DNA repair and replication, chromosomal stability, etc. A variety of post-translational modifications of Histone H3 bring about changes in function[2], phosphorylation of Histone H3 at Ser10[3] and Ser28[4] regulates chromosome condensation during mitosis, Histone H3 at Lys4, Lys9, and Lys14 can be methylated by histone methyltransferases, thereby affecting DNA transcription[5][6].
Storage
PBS (pH 7.4), 0.02% Sodium Azide, 50% Glycerol;
at -20℃ for two years, avoid repeated freeze-thawing.
Shipping
Dry ice (-70℃)
[1]. Workman JL, Kingston RE. Alteration of nucleosome structure as a mechanism of transcriptional regulation[J]. Annual Review of Biochemistry. 1998, 67 (1): 545-79.
[2]. Garcia BA, Barber CM, Hake SB, Ptak C, Turner FB, Busby SA, et al. Modifications of human histone H3 variants during mitosis[J]. Biochemistry. 2005, 44 (39): 13202.
[3]. Kaszás E, Cande WZ. Phosphorylation of histone H3 is correlated with changes in the maintenance of sister chromatid cohesion during meiosis in maize, rather than the condensation of the chromatin[J]. Journal of Cell Science. 2000, 113 ( Pt 18) (18): 3217.
[4]. Goto H, Tomono Y, Ajiro K, Kosako H, Fujita M, Sakurai M, et al. Identification of a Novel Phosphorylation Site on Histone H3 Coupled with Mitotic Chromosome Condensation[J]. Journal of Biological Chemistry. 1999, 274 (36): 25543-9.
[5]. Lee DY, Teyssier C, Strahl BD, Stallcup MR. Role of protein methylation in regulation of transcription[J]. Endocrine Reviews. 2005, 26 (2): 147-70.
[6]. Strahl BD, Allis CD. The language of covalent histone modifications[J]. Nature. 2000, 403 (6765): 41.
