Products

qPCR and qRT-PCR SuperMixes
  • AQ131-01.JPG
AQ131-01.JPG

TransStart® Top Green qPCR SuperMix


Product Details

TransStart® Top Green qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except primer and template. It contains TransStart® TopTaq DNA Polymerase, SYBR Green I, dNTPs, PCR enhancer and stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.

TransStart® TopTaq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.

Storage
at -20°C  in dark for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAQ131-01AQ131-02AQ131-03AQ131-04
TransStart® Top Green qPCR SuperMix1 ml5×1 ml15×1 ml25×1 ml
Passive Reference Dye (50×)40 μl200 μl600 μl1 ml
Nuclease-free Water1 ml5 ml3×5 ml5×5 ml


Citations

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Wei Y , Hu W , Wang Q , et al. Identification, transcriptional and functional analysis of heat-shock protein 90s in banana (Musa acuminata L.) highlight their novel role in melatonin-mediated plant response to Fusarium wilt[J]. Journal of Pineal Research, 2017, 62(1):e12367.

Li P, Du J, Goodier JL, et al. Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion[J]. Nucleic Acids Research, 2017, 45(8):4619-4631.

Sun X, Wang C, Xiang N, et al. Activation of secondary cell wall biosynthesis by miR319-targeted TCP4 transcription factor[J]. Plant Biotechnol . 2017, 15(10):1284-1294. 

Ju Z , Cao D , Gao C , et al. A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants[J]. Plant Physiology, 2017, 173(4):1940-1952.

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