The kit contains a PerfectStartTM Taq DNA Polymerase, optimized dual-cation buffer, SYBR Green I, dNTPs, PCR enhancer and PCR stabilizer. Three monoclonal antibodies bind to the hot-start Taq DNA Polymerase, effectively blocking DNA polymerase activity and inhibiting non-specific amplification at low temperature. qPCR SuperMix is provided at 2×concentration and can be used at 1×concentration by adding template, primers, Universal Passive Reference Dye and nuclease-free water.
Highlights
• Blocking by 3 antibodies; high specificity, sensitivity and amplification efficiency; applicable to a wide range of species.
• Dual-cation buffer enhances specificity and reduces primer-dimer formation.
• Universal Passive Reference Dye for different instruments to correct differences in fluorescence detection between wells due to pipetting errors.
Storage
at -20°C in dark for two years
Shipping
Dry ice (-70 ℃)