Products

Plasmid DNA Purification

EasyPure® EndoLow Plasmid MaxiPrep Kit


Product Details

EasyPure® EndoLow Plasmid MaxiPrep Kit uses alkaline lysis method to extract plasmid DNA from no more than 500 ml of LB E.  coli culture. Unique silica membrane adsorption technique to efficiently bind plasmid DNA. The special TB Buffer can effectively  remove endotoxin. The solution contains indicators that can indicate whether the lysis and neutralization are complete through the  change of color, so as to visualize the procedure. Endotoxin is removed on the column, which is fast and simple to use. The purified  DNA is suitable for a variety of molecular biology applications including restriction enzyme digestion, ligation, transformation,  DNA sequencing, and transfection. The kit can be used 10 times based on a single 100 ml to 200 ml bacterial input. 


Features

• Visual Procedure: solution LB (blue) indicates whether the lysis and neutralization are complete through the change of color, so as  to ensure the quality of plasmid extraction.  

• Fast: the whole procedure can be performed within one hour.  

• Easy to use: on-column rapid endotoxin removal. 

• High yield (up to 4 mg).


Storage
at room temperature (15-25 ℃) under dry conditions for one year.


Shipping
at room temperature.

Product Contents


ComponentEM122-01(10 rxns)
Resuspension Buffer (RB)120 ml
Lysis Buffer (LB, Blue)120 ml
Neutralization Buffer 1 (NB1)120 ml
Activation Buffer (AB)55 ml
ToxinOut Buffer (TB)60 ml
Wash Buffer (WB)25 ml
Elution Buffer (EB)25 ml
RNase A (10 mg/ml )1.2 ml
Maxi-Plasmid Spin Column with Collection Tube10
Push Filter10
50 ml Collection Tube10


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