This product contains TransStart® FastPfu Fly DNA Polymerase, dNTPs and optimized reaction buffer at a concentration of 2×. It has the features of high amplification efficiency and fast amplification speed (≤ 5 kb fragments can achieve 12 kb/min extreme amplification, > 5 kb fragments can achieve 6 kb/min high-speed amplification). The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and Nuclease-free Water for amplification. The amplified product of 2×TransStart®FastPfu Fly PCR SuperMix (-dye) can be cloned directly into pEASY®-Blunt series of vectors. The amplified product of 2×TransStart®FastPfu Fly PCR SuperMix (+dye) can be analyzed by electrophoresis directly, and need to be purified to remove dye when applied in cloning.
• Reduce PCR operation time.
•Avoid contamination caused by multi-step operation.
•TransStart® FastPfu Fly PCR SuperMix offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
•Amplification of genomic DNA fragment up to 15 kb.
•Amplification of plasmid DNA fragment up to 20 kb.
Highlights
Fast, ultra-high fidelity, high specificity, good stability.
Applications
• Ultra high fidelity PCR
• Site-directed mutagenesis
• Blunt end cloning
• Complex templates
• GC/AT-rich templates
• Long fragment amplification
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
